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Iranian Journal of Parasitology، جلد ۱۰، شماره ۱، صفحات ۳۰-۸
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عنوان انگلیسی Evaluation of Dot Immunogold Filtration Assay (DIGFA) By Recombinant Protein EPC1 for Anti- Echinococcus granulosus IgG Antibody.
چکیده انگلیسی مقاله Background: Cystic echinococcosis is an important zoonosis caused by the Echinococcus granulosus with a substantial impact in human and animal health in en-demic areas. The purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method. Methods: Eight dogs were challenged with protoscoleces in order to have posi-tive echinococcosis serum and 2 two-month old puppies were used as uninfected controls. Colloidal gold was prepared by controlled reduction of a boiling solu-tion of chloroauric acid (H [AuCl4]) with sodium citrate and labeled with recom-binant EPC1. Dot immunogold filtration assay (DIGFA) was developed by coat-ing rEPC1 labeled colloidal gold on nitrocellulose membrane. The canine sera, taken three times including, 15, 28 and 35 days post infection were tested. A total of 30 serum samples including 24 sera from 8 infected dogs and 6 sera from 2 puppies were comparatively detected with both DIGFA and ELISA. Results: Gold labeled antigen, showed a dark purple dot with agglutination parti-cles in positive sera and light purple dot without agglutination in negative sera. Among 30 serum samples, 23 were positive and 7 were negative with DIGFA and 24 were positive, 6 were negative with ELISA. Conclusion: DIGFA as a rapid and simple procedure could be utilized in quickly diagnosis of echinococcosis.
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نویسندگان مقاله سمیه کردافشاری | somayeh kordafshari
dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

سید حسین حسینی | seyed hossein hosseini
dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

فاطمه جالوسیان | fatemeh jalousian
dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

معصومه رجبی بذل | masoumeh rajabibazl
dept. of clinical biochemistry, school of medicine, shahid beheshti university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

malcolm جونز | malcolm jones
dept. of parasitology, school of veterinary sciences, queensland university, queensland, australia.


فاضله اعتبار | fazeleh etebar
dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/355
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-349967.pdf
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زبان مقاله منتشر شده en
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