این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۱۰، شماره ۱، صفحات ۴۶-۵۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Morphological and molecular discrimination of fasciola species isolated from domestic ruminants of urmia city, iran.
چکیده انگلیسی مقاله Background: The trematodes of the genus Fasciola (the liver flukes) are among the well-known instances of food-borne parasites worldwide. Differentiation of Fasciola species is important because of their different transmission and epidemio-logical characteristics. The current study was undertaken to discriminate Fasciola species in the domestic ruminants of Urmia city, Iran. Methods: Adult flukes were isolated from the naturally infected livers of the slaughtered water buffaloes and sheep. The flukes were initially identified based on morphological and morphometric parameters. A 618-bp-long fragment of the 28SrRNA gene of Fasciola was amplified by polymerase chain reaction (PCR). The amplified fragment was digested by Dra II or Ava II enzymes for a restriction frag-ment length polymorphism (RFLP) analysis and sequenced for the phylogenetic tree construction. Results: Based on the morphometric examination, the flukes belonged to F. he-patica , F. gigantica and an intermediate Fasciola form. The PCR-RFLP analysis was able to differentiate F. hepatica from F. gigantica . While the phylogenetic reconstruc-tion justified, to some extent, the morphological diagnosis, it failed to segregate F. hepatica from F. gigantica identified in this and the previous studies. Conclusion: To resolve fully the problem of taxonomy and evolution in Fasciola species, employing a broad range of molecular and morphological approaches is necessary. This is crucial for epidemiological surveys and successful clinical man-agement of their infection.
کلیدواژه‌های انگلیسی مقاله 28SrRNA gene,Liver flukes,Morphology,PCR-sequencing,RFLP
نویسندگان مقاله محمد یخچالی | mohammad yakhchali
dept. of pathobiology, faculty of veterinary medicine, urmia university, urmia, iran.

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

رضا ملک زاده viayeh | reza malekzadeh viayeh
lake urmia research institute, urmia university, urmia, iran.

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

عباس ایمانی باران | abbas imani baran
dept. of pathobiology, faculty of veterinary medicine, tabriz university, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

کریم مردانی | karim mardani
dept. of food hygiene and quality, faculty of veterinary medicine, urmia university, urmia, iran.

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/353
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-349969.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Articles
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