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Iranian Journal of Parasitology، جلد ۱۰، شماره ۱، صفحات ۶۹-۷۷
عنوان فارسی
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عنوان انگلیسی Development of an Indirect ELISA Using Different Fragments of Recombinant Ncgra7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo.
چکیده انگلیسی مقاله Background: Dense granules are immunodominant proteins for the standardi-zation of immunodiagnostic procedures to detect neosporosis. In the presented study different fragment of a dense-granule protein was evaluated for serodiagno-sis of Neospora caninum in cattle and water buffalo. Methods: NcGRA7, from N. caninum tachyzoites was amplified. PCR product and pMAL-c2X plasmid were digested with EcoR1 restriction enzyme and ex-pressed in Escherichia coli to evaluate its competence for detection of anti- N. cani-num antibodies with ELISA in comparison with commercial IDEXX ELISA. Fur-thermore, 230 sera of presumably healthy cattle and water buffaloes (108 cattle and 122 water buffaloes) were analyzed by both tests to determine the agreement of these two procedures. Results: Sensitivities and specificities of NcGRA7-based ELISA were 94.64% and 90.38% respectively using sera of cattle , but were 98.57% and 86.54% in the case of buffaloes respectively . A good correlation between the results of IDEXX ELISA and ELISA based on recombinant NcGRA7 for detecting N. caninum an-tibodies was appeared . Analyzing by Mc Nemar´s showed that NcGRA7-based ELISA has acceptable capability to differentiate the positive results in comparison with IDEXX ELISA. Conclusion: NcGRA7-based ELISA considering utilized new fragment of ge-nomic DNA is a good tool for serodiagnosis of anti- N. caninum antibodies for screening and epidemiological purposes on cattle herd and water buffaloes as well.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله حسین حمیدی نجات | hossein hamidinejat
dept. of pathobiology, veterinary faculty, shahid chamran university of ahvaz, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

مسعود رضا سیفی آباد شاپوری | massoud reza seifi abad shapouri
dept. of pathobiology, veterinary faculty, shahid chamran university of ahvaz, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

محمد مهدی نام آوری | mohammad mehdi namavari
razi vaccine and serum research institute, shiraz branch, shiraz, iran.

سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)

پرویز شایان | parviz shayan
dept. of parasitology, veterinary faculty, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

مرضیه کفایت | marzieh kefayat
dept. of parasitology, shahid chamran university of ahvaz, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/350
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-349972.pdf
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