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Iranian Journal of Parasitology، جلد ۱۰، شماره ۱، صفحات ۸۷-۹۵
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عنوان انگلیسی Molecular characterization of eimeria species naturally infecting egyptian baldi chickens.
چکیده انگلیسی مقاله Background: Coccidiosis is a serious protozoal disease of poultry. The iden-tification of Eimeria species has important implications for diagnosis and con-trol as well as for epidemiology. The molecular characterization of Eimeria spe-cies infecting Egyptian baladi chickens was investigated. Methods: Eimeria species oocysts were harvested from intestines of naturally infected Egyptian baldi chickens. The morphometry characterization of oocysts along with COCCIMORPH software was done. The DNA was extracted ini-tially by freezing and thawing then the prepared samples was subjected to commercial DNA kits. The DNA products were analyzed through conven-tional polymerase chain reaction by using amplified region (SCAR) marker. Results: The PCR results confirmed the presence of 7 Eimeria species in the examined fecal samples of Egyptian baldi breed with their specific ampilicon sizes being E. acervulina (811bp), E. brunette (626bp), E. tenella (539bp), E. max-ima (272bp), E. necatrix (200bp), E. mitis (327bp) and E. praecopx (354bp). A sequencing of the two most predominant species of Eimeria was done, on E. tenella and E. máxima . Analysis of the obtained sequences revealed high identi-ties 99% between Egyptian isolates and the reference one. Similarly, E. maxima isolated from Egyptian baldi chickens showed 98% nucleotide identities with the reference strain. Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one. The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence. Conclusion: This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens.
کلیدواژه‌های انگلیسی مقاله Chickens,DNA,Eimeria species,Nucleotide,PCR,Sequencing
نویسندگان مقاله سحر m gadelhaq | sahar m gadelhaq
parasitology department, faculty of veterinary medicine, beni-suef university, egypt.


waleed m arafa | waleed m arafa
parasitology department, faculty of veterinary medicine, beni-suef university, egypt.


shawky m aboelhadid | shawky m aboelhadid
parasitology department, faculty of veterinary medicine, beni-suef university, egypt.
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/348
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-349974.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Articles
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