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Medical Laboratory Journal، جلد ۱۹، شماره ۱، صفحات ۰-۰
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Molecular evaluation of Leishmania species in negative ulcer smears from patients suspected of having cutaneous leishmaniasis referred to health center of Aq Qala city during 2019-2020
چکیده انگلیسی مقاله Background and objectives: Cutaneous leishmaniasis (CL) is a prevalent infectious zoonotic disease between human and animals. Golestan province is one of the important centers of CL in Iran. Current method for detecting Leishmania parasite in patients is Giemsa-stained direct smear from skin ulcers; however, PCR is a strongly recommended method for epidemiological studies. The aim of this study is to investigate Leishmania species in negative ulcer smears from patients suspected of having CL referred to Aq Qala health center using multiplex nested PCR method. 
Methods: This study was performed on 72 negative ulcer smears from patients suspected of having CL referred to Aq Qala health center, Golestan province, northeastern Iran, during August 2019 to April 2020 using multiplex nested PCR method to detect Leishmania major and Leishmania tropica species.
Results: Out of 72 samples, 4 (5.55%) samples were positive by multiplex nested PCR. Moreover, all positive samples are related to Leishmania major species.
Conclusion: Detecting of Leishmania species is strongly recommended in negative ulcer smears from patients suspected of having CL using multiplex nested PCR method.
کلیدواژه‌های انگلیسی مقاله Cutaneous leishmaniasis, Negative ulcer smear, Leishmania major, Leishmania tropica, multiplex nested PCR
نویسندگان مقاله | Fatemeh Livani
Golestan University of Medical Sciences, Gorgan, Iran


| Faramarz Koohsar
Golestan University of Medical Sciences, Gorgan, Iran


| Farideh Tohidi
Golestan University of Medical Sciences, Gorgan, Iran


| Mitra Sharbatkhori
Golestan University of Medical Sciences, Gorgan, Iran


| Roghiyeh Faridnia
Golestan University of Medical Sciences, Gorgan, Iran


| Ayeneh hajieh Pangh
Golestan University of Medical Sciences, Gorgan, Iran


| Mehdi Khoshrou
Golestan University of Medical Sciences, Gorgan, Iran


| Hamed Kalani
Golestan University of Medical Sciences, Gorgan, Iran
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-1375-2&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده انگل شناسی
نوع مقاله منتشر شده Research Article
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