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Iranian Journal of Medical Sciences، جلد ۳۹، شماره ۲، صفحات ۱۰۷-۰
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عنوان انگلیسی Small Molecule-BIO Accelerates and Enhances Marrow-Derived Mesenchymal Stem Cell in Vitro Chondrogenesis
چکیده انگلیسی مقاله Background: Hyaline cartilage defects exhibit a major challenge in the field of orthopedic surgery owing to its limited repair capacity. On the other hand, mesenchymal stem cells (MSCs) are regarded as potent cells with a property of cartilage regeneration. We aimed to optimize marrow-derived MSC chondrogenic culture using a small bioactive molecule referred to as BIO. Methods: MSCs from the marrow of NMRI mice were extracted, culture-expanded, and characterized. Micro-mass culture was then established for chondrogenic differentiation (control group). The cultures of MSC in chondrogenic medium supplemented with 0.01, 0.05, 0.1, and 1 µM BIO were taken as the experimental groups. Cartilage differentiation was examined by both histological sections and real-time PCR for Sox9, aggrecan, and collagen II at different time points. Moreover, the involvement of the Wnt pathway was investigated. Results: Based on histological sections, there was seemingly more intense metachromatic matrix produced in the cultures with 0.01 µM BIO. In this experimental group, cartilage-specific genes tended to be upregulated at day 14 compared to day 21 of the control group, indicating the accelerating effect of BIO on cartilage differentiation. Overall, there was statistically a significant increase (P=0.01) in the expression level of cartilage-specific genes in cultures with 0.01 µM BIO (enhancing effects). These upregulations appeared to be mediated through the Wnt pathway evident from the significant upregulation of T-cell factor and beta-catenin molecules (P=0.01). Conclusion: Taken together, BIO at 0.01 µM could accelerate and enhance in vitro chondrogenesis of mouse marrow-derived MSCs.
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نویسندگان مقاله محمدرضا باغبان اسلامی نژاد | mohamadreza baghaban eslaminejad
department of stem cell and developmental biology at cell science research center, royan institute for stem cell biology and technology, acecr, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

نسرین فلاح | nasrin fallah
department of developmental biology, university of science and culture, tehran, iran

سازمان اصلی تایید شده: دانشگاه علم و فرهنگ (University of science and culture)

نشانی اینترنتی http://ijms.sums.ac.ir/index.php/IJMS/article/view/588
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/147/article-147-350751.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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