این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۷، شماره ۳، صفحات ۳۶۶-۳۷۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Phenotypic, genotypic characterization and antimicrobial resistance profiling of uropathogenic Escherichia coli in a tertiary care hospital, Puducherry, India
چکیده انگلیسی مقاله Background and Objectives: Uropathogenic Escherichia coli (E. coli) (UPEC) accounts for 70-95% of community-acquired urinary tract infections (UTIs) and a significant proportion of nosocomial UTIs. This study aimed to characterize the phenotypic and genotypic characteristics of E. coli isolates from symptomatic UTI patients and evaluate their antimicrobial susceptibility patterns. Materials and Methods: A hospital-based observational study was conducted at Aarupadai Veedu Medical College and Hospital, Puducherry, India, from August 2022 to April 2024. A total of 106 UPEC isolates were obtained from symptomatic UTI patients. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer method, and virulence genes (hlyA, fimH, papC) were detected using PCR. Results: The mean age of patients was 49.7 years, with a female predominance (69.8%). Diabetes mellitus was the most common comorbidity (29.2%). Fever (60.4%) and dysuria (38.7%) were the most common symptoms. AST showed high susceptibility (>90%) to amikacin, nitrofurantoin, meropenem, and piperacillin/tazobactam, while >60% resistance was observed to cefotaxime and ceftazidime. Phenotypically, 30.2% of the isolates produced mannose-resistant hemagglutinins, and 17.9% produced hemolysin. ESBL production was found in 46.3%. Biofilm production was moderate in 65.1%, weak in 30.2% and strong in 4.7% and significantly correlated with multidrug resistance (p<0.05). Genotypically, 80.2% had fimH, 51.9% had papC and 20.8% had hlyA. papC was associated with reduced cefotaxime susceptibility (p<0.05). Conclusion: The study highlights the significance of phenotypic and genotypic characterization in understanding UPEC virulence and resistance patterns, and emphasizes the need for targeted empiric therapy to improve UTI management.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Sherief Shebeena
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Latha Ragunathan
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Kavitha Kannaiyan
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Thiyagarajan Sanjeevi
Department of Medical Biotechnology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Pramodhini Subramanian
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Aravind Sasi
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Jaishma Rajni
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Jenifer Raj
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India


| Mohamed Asarudeen
Department of Microbiology, Aarupadai veedu Medical College and Hospital, Vinayaka Mission&#x2019;s Research Foundation (DU), Kirumampakkam, Puducherry, India
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5293
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات