این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Medical Signals and Sensors، جلد ۱۴، شماره ۷، صفحات ۱۰-۴۱۰۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Designing an Electrochemical Biosensor Based on Voltammetry for Measurement of Human Chorionic Gonadotropin
چکیده انگلیسی مقاله Abstract Background:  Human chorionic gonadotropin (hCG) is a polypeptide hormone synthesized during pregnancy and is also upregulated in some pathologic conditions such as certain tumors. Its measurement is essential for diagnosing pregnancy and malignancies. Despite numerous attempts to introduce an accurate method capable of detecting hCG levels, several limitations are found in previous techniques. This study aimed to address the limitations of current hCG assay methods by designing an electrochemical biosensor based on voltammetry for the rapid, selective, inexpensive, and sensitive measurement of hCG levels. Methods:  A carbon paste electrode was prepared and functionalized by para-aminobenzoic acid. The primary anti-B-hCG monoclonal antibody was immobilized on the electrode surface by activating the carboxyl groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide solutions. The study also involved optimizing parameters such as the time for primary antibody fixation, the time for hCG attachment, and the pH of the hydrogen peroxide solution to maximize the biosensor response. Different concentrations of hCG hormone were prepared and loaded on the electrode surface, the secondary antibody labeled with HRP enzyme was applied, thionine in phosphate-buffered saline solution was placed on the electrode surface, and the differential pulse electrical signal was recorded. Results:  The linear range ranged from 5 to 100 mIU/ml, and the limit of detection was calculated as 0.11 mIU. The relative standard deviation was 3% and 2% for five repeated measurements of commercial standard samples with concentrations of 2 and 20 mIU/mL, respectively. The percent recovery was obtained from 98.3% to 101.5%. Conclusion:  The sensor represents a promising advancement in hCG level measurement, offering a potential solution to overcome the existing limitations in current diagnostic strategies. Simple and inexpensive design, detecting hCG in its important clinical range during early pregnancy, and successful measurement of hCG in real serum samples are the advantages of this sensor.
کلیدواژه‌های انگلیسی مقاله Biosensor,electrochemistry,voltammetry,B-human chorionic gonadotropin
نویسندگان مقاله | Mohammad Samare-Najaf
3-Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran 4-Department of Pathology, Molecular Pathology and Cytogenetics Division, Shiraz University of Medical Sciences, Shiraz, Iran


| Amirreza Dehghanian
Applied Cellular and Molecular Research Center, Kerman University of Medical Sciences, Kerman, Iran


| Gholamreza Asadikaram
Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran


| Maryam Mohamadi
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Morteza Jafarinia
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran


| Amir Savardashtaki
Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran


| Afrooz Afshari
Infertility Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran


| Sina Vakili


نشانی اینترنتی http://jmss.mui.ac.ir/index.php/jmss/article/view/722
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات