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Physiology and Pharmacology، جلد ۲۹، شماره ۴، صفحات ۴۰۸-۴۲۳
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چکیده فارسی مقاله
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عنوان انگلیسی Evaluation of the effect of biodegradable dressing prepared from chitosan and external microvesicles of Bifidobacterium bifidum on burn wound healing in male Wistar rats
چکیده انگلیسی مقاله Introduction: The burn wound healing process is multi-variable, and various factors are involved. Chitosan-based dressings are acceptable materials for wound healing. PProbiotics and postbiotics can in-crease the effectiveness of these dressings. This study aimed to investigate the effect of a biodegradable dressing prepared from chitosan and external microvesicles of Bifidobacterium bifidum on burn wound healing in male Wistar rats. Methods: Bifidobacterium bifidum was cultured, the activated crude supernatant (ACS) was separated, and the microvesicles were isolated with the help of Ultra-centrifugation at 150,000×g at 4°C for 30 minutes. Chitosan films containing and without microvesicles were prepared using a heater stirrer at 29°C. The effectiveness of the membranes was evaluated. For this purpose, 60 male rats with second-degree burns were randomly divided into 5 groups (n=12) and were treated for 21 days. The process of wound healing was examined macroscopically and microscopically (wound histology and evaluation of gene expression of cytokines interleukin-8, interleukin-10, and VEGF) on the days 3, 7, 14 and 21. The obtained data were analyzed by IBM SPSS.21 software and using Kolmogorov-Smirnov, Kruskal-Wallis, and Mann-Whitney tests with p ≤ 0.05. Results: The average diameter of microvesicles was 174 ± 52nm. The thickness of the layers was 2mm. The wounds of the group post-biotic and positive control were closed on the 14th day. Collagen production and epithelialization, as well as inflammation control in the treatment groups, were higher than negative control. Conclusion: The use of chitosan membranes, particularly those enhanced with ACS and microvesicles, represents a significant advancement in wound care by inflammation control, offering a promising strategy for improving the healing of burn wounds.
کلیدواژه‌های انگلیسی مقاله Wound healing, Microvesicle, Chitosan, Bifidobacterium bifidum
نویسندگان مقاله | Seyed Amin Mousavinezhad
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran


| Naser Harzandi
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran


| Azam Marjani
1-Department of Chemistry, Arak Branch, Islamic Azad University, Arak, Iran2-Halal Reserch Center of IRI, Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran


| Parvaneh Jafari
Department of Microbiology, Arak Branch, Islamic Azad University, Arak, Iran
نشانی اینترنتی http://ppj.phypha.ir/browse.php?a_code=A-10-2456-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Clinical Physiology/Pharmacology
نوع مقاله منتشر شده Experimental research article
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