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Acta Medica Iranica، جلد ۶۳، شماره ۶، صفحات ۳۲۴-۳۲۹
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عنوان انگلیسی Azole Resistance and CYP51A/B Mutations in Aspergillus Clinical Isolates Before and During the COVID-19 Pandemic: A Molecular Surveillance Study
چکیده انگلیسی مقاله We conducted a cross-sectional study to (I) determine the relative frequency of antifungal-resistant Aspergillus clinical isolates, (II) address changes in susceptibility to available antifungals in patients infected with Aspergillus spp. with COVID-19, and (III) determine mutations in the CYP51A and CYP51B genes of Aspergillus spp. Isolated from the clinical specimens. A total of 30 fungal species were enrolled in the study. The antifungal activities of itraconazole and voriconazole were assessed using azole-containing agar media in Petri dishes. After identifying resistance in the isolates, the CYP51A and CYP51B gene regions were sequenced using the designed primers, and mutations were identified. To amplify CYP51A and CYP51B, primers with the specified sequences were used. Genomic DNA from 22 azole-resistant Aspergillus isolates was amplified using the CYP51-A and CYP51-B gene primers. 12/22 (54.54%) azole-resistant A. flavus isolates with the Tandem Repeat (TR34)/L98H (leucine-to-histidine substitution) mutation, MICs above the CLSI Epidemiological Cutoff Value. One carried the F46Y /TR34. 5/22 azole non-WT A. fumigatus isolates, CYP51-A analysis revealed that M220I, S297T/ TR34/L98H mutations, 4 A.orezea isolates had C498T/TR34 at a CYP51-B gene. Antifungal susceptibility testing should be performed when possible, and efficient systems must be implemented to monitor the evolution of newly introduced azole-resistant Aspergillus spp. In addition, these data are useful for clinicians to understand the incidence of azole resistance, enabling optimal management of affected patients and helping choose the right solution for infection management.
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نویسندگان مقاله | Mohammadrafi Khorgami
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.


| Shirin Sayyahfar
Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.


| Negar Omidi
Research Institute, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.


| Maryam Moradian
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.


| Nikta Aliabadi
Department of Microbiology, Islamic Azad University, Tehran Branch, Tehran, Iran.


| Pegah Tamimi
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.


| Aliasghar Ghaderi
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.


| Hengameh Soudi
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran


| Fatemeh Naderi
Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.


| Mahsa Fattahi
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Children's Medical Center, Pediatrics Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran.


| Fatemeh Omidi
Department of Cardiology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
نشانی اینترنتی https://acta.tums.ac.ir/index.php/acta/article/view/11658
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