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Iranian Journal of Otorhinolaryngology، جلد ۳۸، شماره ۲، صفحات ۱۱۷-۱۲۴
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عنوان انگلیسی Pepsin-induced Differential IL-6 Expression in Laryngeal and Laryngopharyngeal Tissue: Non-invasive Rat Model for Laryngopharyngeal Reflux
چکیده انگلیسی مقاله Introduction: Laryngopharyngeal reflux is a common condition caused by exposure of the upper aerodigestive tract to gastroduodenal content, resulting in inflammation to the area. Despite the condition being well documented in the human population, the literature on animal models for laryngopharyngeal reflux is currently limited. The aim of this study is to create a non-invasive model of laryngopharyngeal reflux using rats to investigate changes in IL-6 expression in the laryngeal and laryngopharyngeal regions.Materials and Methods: Male Wistar rats, ranging from ages 10-14 weeks, were exposed to 0.5% bovine pepsin dissolved in artificial gastric juice three times daily for 5-15 days via a modified IV cannula in several volumes          (0 mL, 0.1 mL, 0.15 mL, and 0.2 mL) according to the treatment groups. Subsequently, laryngeal and laryngopharyngeal tissue IL-6 concentrations were measured through ELISA on allotted days of sacrifice.Results: Significant differences in laryngeal and laryngopharyngeal tissue IL-6 concentration were measured     (p = 0.005 and p = 0.020 respectively). Laryngeal expression of IL-6 was related to the volume administered (p = 0.007) but not to the duration of exposure (p = 0.338), with no significant interaction between duration of exposure and administered volume (p = 0.515). On the other hand, laryngopharyngeal expression of IL-6 was related to the duration of exposure (p< 0.001) but not to the volume administered (p = 0.521) with significant interaction between duration and volume (p < 0.001).Conclusion: The study established the feasibility of using a non-invasive rat model for studying laryngopharyngeal reflux. Additionally, the study demonstrated the differential responses of IL-6 to the addition of the acid-pepsin insult, with dose-dependent IL-6 responding more in laryngeal tissue and time-dependent IL-6 responding in laryngopharyngeal tissue.
کلیدواژه‌های انگلیسی مقاله Animal model, Laryngopharyngeal Reflux, Tissue IL-6
نویسندگان مقاله | Adelien Adelien
Department of Otorhinolaryngology and Head and Neck Surgery, Faculty of Medicine, Sriwijaya Universty Indonesia.


| Irfannuddin Irfannuddin
Department of Physiology, Faculty of Medicine, Sriwijaya University Indonesia.


| Hardi Darmawan
Department of Physiology, Faculty of Medicine, Sriwijaya University Indonesia.


| Agus Surono
Department of Otorhinolaryngology and Head and Neck Surgery, Faculty of Medicine, Gadjah Mada University Indonesia.


| Krisna Murti
Department of Anatomical Pathology, Faculty of Medicine, Sriwijaya University Indonesia.


| Debby Harahap
Department of Pharmacology, Faculty of Medicine, Sriwijaya University Indonesia.


| Elvie Zulka
Department of Otorhinolaryngology and Head and Neck Surgery, Faculty of Medicine, Sriwijaya University Indonesia.
نشانی اینترنتی https://ijorl.mums.ac.ir/article_27388.html
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