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Iranian Journal of Microbiology، جلد ۱۸، شماره ۱، صفحات ۱-۱۳
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عنوان انگلیسی A comparative analysis of CRISPR systems, virulence factors, and antibiotic resistance genes in carbapenem-sensitive and carbapenem-resistant Klebsiella pneumoniae
چکیده انگلیسی مقاله Background and Objectives: Klebsiella pneumoniae is a major cause of healthcare-associated infections, particularly in immunocompromised patients. This study compares the CRISPR systems, virulence factors, and antibiotic resistance genes in carbapenem-sensitive (CSKP) and carbapenem-resistant (CRKP) clinical isolates. Materials and Methods: Carbapenemase-producing isolates were identified by mCIM/eCIM. PCR and RT-qPCR detected key genes, including cas3, involved in CRISPR-Cas function. In silico analyses included STRING for protein interactions, CRISPRCasdb for CRISPR subtype distribution, and Phyre2/AlphaFold for cas3 structure prediction. Results: Among the isolates, 35.2% were resistant to carbapenems. Among CRKP strains, high prevalence of bla-NDM-1 (82%) and bla-OXA-48 (64%) was observed. The cas3 expression was significantly upregulated in resistant isolates (P = 0.002). CRISPR subtype I-E was identified in 16% of CRKP and 36% of CSKP isolates. Structural-functional analysis supported the integrity of Cas3 and revealed interactions with regulatory and iron acquisition proteins. Statistically significant differences in virulence and resistance gene profiles were found between CRKP and CSKP groups (P < 0.05). Conclusion: This study highlights key differences between CRKP and CSKP isolates, particularly in CRISPR-Cas systems, resistance, and virulence. The findings suggest that cas3 plays a critical role in genomic adaptation and resistance mechanisms in K. pneumoniae, offering insights for future therapeutic strategies.
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نویسندگان مقاله | Samira Saedi
Department of Medical Microbiology, Khalkhal University of Medical Sciences, Khalkhal, Iran


| Javad Nezhadi
Department of Medical Microbiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran


| Hadi Feizi
Deparment of Medical Microbiology, Alinasab Hospital, Sccial Security Organization, Tabriz, Iran


| Mohammad Yousef Memar
Infectious and Tropical Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Vahid Arefi
Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran


| Hiva Kadkhoda
Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Microbiology, Mahabad Faculty of Medical Sciences, Urmia University of Medical Sciences, Urmia, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5720
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