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Iranian Journal of Microbiology، جلد ۱۸، شماره ۱، صفحات ۲۳-۳۱
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عنوان انگلیسی Detection and distribution of carbapenemase-encoding genes in clinical Klebsiella pneumoniae isolates from Kayseri, Türkiye
چکیده انگلیسی مقاله Background and Objectives: Carbapenem Resistance Klebsiella pneumonia (CRKP ), mostly caused by carbapenemase enzymes, poses a serious public health threat due to limited treatment options. This study aimed to genotypically identify carbapenemase-encoding genes in CRKP isolates recovered from fecal swabs and to correlate these genotypes with phenotypic antibiotic resistance profiles. Materials and Methods: In this study, fecal samples from 150 hospitalized patients were screened for K. pneumoniae. Phenotypic testing included the Phoenix automated system, CHROMagar KPC, biochemical tests, and disk diffusion assays. Genotypic analysis was performed using the BD MAX Checkpoint CPO PCR test, marking its first use in Kayseri, Türkiye, to detect carbapenemase genes in this pathogen. Results: Out of 150 fecal samples, 47 tested positive for K. pneumoniae, with 28 (59.6%) identified as carbapenem-resistant (CRKP). Molecular analysis identified five distinct carbapenemase gene patterns among these resistant isolates. The most prevalent gene, blaOXA-48, was found alone in 60.7% of CRKP isolates, followed by blaNDM in 3.6%; blaKPC was not detected. Co-occurrence of genes was observed as follows: blaOXA-48/blaNDM (14.3%), blaOXA-48 with blaVIM/blaIMP (10.7%), and blaOXA-48 with blaNDM and blaVIM/blaIMP (10.7%) in CRKP clinical isolates. Conclusion: The study found that blaKPC was consistently absent, while blaOXA-48 was highly prevalent and exhibited co-occurrences of carbapenemase genes. It underscored the need for strict hospital surveillance and effective infection control to prevent the spread of CRKP strains. Rapid molecular methods, such as the BD MAX multiplex PCR, have shown promise in accurately and efficiently identifying carbapenemase genes.
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نویسندگان مقاله | Thowiba Jameel
Department of Biology, Graduate School of Natural and Applied Science, Erciyes University, Kayseri, Türkiye; Department of Biology, Faculty of Science, Tikrit University, Tikrit, Iraq


| Abdurrahman Ayvaz
Department of Biology, Faculty of Science, Erciyes University, Kayseri, Türkiye


| Pınar Sağıroğlu
Department of Medical Microbiology, School of Medicine, Erciyes University, Kayseri, Türkiye


| Mustafa Atalay
Department of Medical Microbiology, School of Medicine, Erciyes University, Kayseri, Türkiye
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5476
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