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Iranian Journal of Microbiology، جلد ۱۸، شماره ۱، صفحات ۹۱-۱۰۱
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عنوان انگلیسی Comparative evaluation of outer membrane protein and whole cell antigen vaccine against avian pathogen Escherichia coli infection in broiler chicken
چکیده انگلیسی مقاله Background and Objectives: Present study was aimed at assessing protective efficacy of outer membrane protein (OMP) vaccine in comparison to inactivated whole cell antigen vaccine after challenge with homologous serogroup (O2) of avian pathogenic Escherichia coli in broiler chickens. Materials and Methods: The outer membrane proteins were extracted by sarcosyl method and protein concentration was determined by nanodrop spectrophotometer. The study comprised of 120 birds divided into 6 groups. The birds were subcutaneously immunized twice with primary vaccine in the first week followed by booster vaccine in second week. Results: The protection rate of 82% was found in whole cell inactivated antigen, 91% in OMP vaccine and 27% among the unvaccinated group. The antibody (IgG) response was found significantly higher in OMP vaccine group than whole cell antigen group. In unvaccinated groups chicks, the antibody titer never reached to the protective level till the termination of experiment. The bacteria were re-isolated from the infected broiler chickens for the confirmation of induced infection and were characterized using standard cultural and biochemical tests belonging to O2 serogroup. Conclusion: Our study demonstrated that the outer membrane protein (OMP) vaccine provided significantly higher protection (91%) and antibody response compared to the inactivated whole cell antigen vaccine (82%) against Escherichia coli O2 infection in broiler chickens. Birds vaccinated with OMP exhibited fewer pathological lesions and a stronger immune response. The findings underscore the potential of OMP-based vaccines as a safer, more immunogenic alternative for controlling colibacillosis.
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نویسندگان مقاله | Basharat Wani
Department of Veterinary Pathology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Shayaib Ahmad Kamil
Department of Veterinary Pathology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Showkat Shah
Department of Veterinary Pathology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Masood Saleem Mir
Department of Veterinary Pathology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Mir Nadeem Hassan
Depatment of Veterinary Microbiology & Immunology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Zahid Amin Kashoo
Depatment of Veterinary Microbiology & Immunology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Mudasir Ali Rather
Department of Veterinary Public Health, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Majid Shafi
Department of Veterinary Pathology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Zahoor Ahmad Wani
Department of Veterinary Parasitology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Nadeem Shabir
Department of Animal Biotechnology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Nazli Tizro
Department of the Environment, College of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Akeel Bashir
Department of Veterinary Pathology, FVSc & AH, Shuhama, SKUAST-Kashmir, India


| Atif Wani
Department of Veterinary Biochemistry, FVSc & AH, Shuhama, SKUAST-Kashmir, India
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5272
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