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Iranian Journal of Microbiology، جلد ۱۸، شماره ۱، صفحات ۱۶۵-۱۷۱
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عنوان انگلیسی Aflatoxin-producing Aspergillus flavus from food samples in Nepal
چکیده انگلیسی مقاله Background and Objectives: Maize and peanuts, staple foods in Nepal, are highly susceptible to fungal contamination, particularly with aflatoxin-producing Aspergillus flavus. This study aimed to detect aflatoxin-producing A. flavus in maize and peanut samples collected from different regions of Nepal. Materials and Methods: A total of 80 maize and 20 peanut samples were collected randomly from markets and households. Disinfected samples were inoculated on Potato Dextrose Agar and incubated at 28°C for 48 hours. A. flavus was identified by conventional cultural methods. Aflatoxin production was screened on Aspergillus Differential Medium (ADM) and confirmed through Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Results: Fungal contamination was detected in 67.0% of samples, with a significantly higher rate in household-stored samples (p = 0.00039). A. flavus was the predominant species, particularly in maize (72.5%). Of 50 A. flavus isolates, 15 (30%) were aflatoxin positive on ADM, with 11 (73.3%) being confirmed by TLC. HPLC revealed AFB1 as the most prevalent in both maize and peanuts, while AFB2 was restricted to maize. Conclusion: Maize and peanuts are highly susceptible to contamination with aflatoxin producing A. flavus in Nepal, particularly in household-stored samples, emphasizing significant food safety concerns.
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نویسندگان مقاله | Suprina Sharma
Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal


| Dev Joshi
Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal


| Pramod Poudel
Central Department of Biotechnology, Tribhuvan University, Kirtipur, Nepal


| Supriya Sharma
Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal


| Tika Karki
Department of Biotechnology, Kathmandu University, Kavre, Nepal


| Reshma Tuladhar
Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5680
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