| چکیده انگلیسی مقاله |
Objective(s): Preimplantation embryo culture is a critical phase in assisted reproductive technologies. The use of small molecules to modulate signaling pathways has emerged as a strategy to improve embryo viability. SB431542 inhibits the TGF-β pathway, while PD0325901 inhibits the MEK/ERK pathway. This study investigates whether their combined application affects embryonic development and cellular composition during early culture, independent of stem cell derivation.Materials and Methods: To assess the effects of SB431542 and PD0325901 on early embryo development, mouse embryos at 2PN, two-cell, and eight-cell stages were collected in vivo and cultured in LIF-supplemented medium containing both molecules until the blastocyst stage. Embryo quality, apoptosis, and developmental progression were assessed using differential staining, TUNEL assay, and continuous monitoring throughout the culture period.Results: Embryos treated with SB431542 and PD0325901 showed a slightly higher rate of blastocyst formation than controls, though the difference was not statistically significant. Degeneration and apoptosis rates were also comparable between groups. However, treated embryos exhibited a significant reduction in ICM cells at early stages, suggesting compromised inner cell mass development. These findings indicate that dual inhibition may not enhance embryo quality under the tested conditions.Conclusion: SB431542 and PD0325901, when applied during preimplantation embryo culture, do not significantly improve developmental progression or reduce degeneration and apoptosis. The observed decrease in ICM cells suggests limited benefit for embryo culture protocols designed to support early development. Further studies are needed to evaluate their relevance in stem cell derivation contexts. |
| نویسندگان مقاله |
| Faezah Mohammadi Ghazani
Faculty of Development of Biology, University of Science and Culture, Tehran, Iran|Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
| Fatemeh Shirvanizadeh
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
| Seyedeh-Nafiseh Hassani
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
| Azam Dalman
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
| Nahid Nasiri
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran|Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
| Maryam Zarei
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
| Saman Maroufizadeh
Department of Biostatistics and Epidemiology, School of Health, Guilan University of Medical Sciences, Rasht, Iran
| Poopak Eftekhari Yazdi
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
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