| چکیده انگلیسی مقاله |
Background and purpose: Breast cancer remains one of the leading causes of cancer-related deaths among women worldwide. The HER2 protein, an epidermal growth factor receptor, is overexpressed in approximately 25% of breast cancer cases, making it a key target for therapeutic strategies. Pertuzumab is a monoclonal antibody used in combination with trastuzumab and docetaxel for the treatment of metastatic HER2-positive breast cancer; it is also used in the same combination as a neoadjuvant in early HER2-positive breast cancer. BmK AGAP, a venom-derived protein, has also shown potential in inducing apoptosis in cancer cells, thereby expanding targeted treatment options.
Materials and methods: This theoretical study employed bioinformatics tools to design an immunotoxin combining a HER2-specific scFv with the BmK AGAP toxin, linked via a peptide linker. Comprehensive analyses were conducted to evaluate the construct's secondary structure, physicochemical properties, and solubility. Subsequently, the tertiary structure of the immunotoxin was modeled, refined, and analyzed. Protein-protein docking was performed to assess the binding affinity to the target receptor, and molecular dynamics simulations were carried out to evaluate the structural stability of the immunotoxin.
Results: The analytical findings suggest that the immunotoxin is a stable protein exhibiting an appropriate structural configuration and no allergenic characteristics. It demonstrates the ability to bind to the HER2 receptor. The synthesized protein molecule shows promise as an immunotoxin targeting HER2-positive breast cancer.
Conclusion: In addition to the favorable results obtained from bioinformatics studies, it is necessary to perform various immunological experiments in vitro and in vivo to confirm the effectiveness of the designed immunotoxin.
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| نویسندگان مقاله |
صدف آزدو | Sadaf Azdoo
Assistant Professor, School of Dentistry, Arak University of Medical Sciences, Arak, Iran
استادیار، دانشکده دندانپزشکی، دانشگاه علوم پزشکی اراک، اراک، ایران
سمیرا مختاری | Samira Mokhtari
Msc in Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
کارشناسی ارشد بیوشیمی بالینی، گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
نوید نظافت | Navid Nezafat
Assistant Professor, Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
استادیار، مرکز تحقیقات علوم دارویی، دانشگاه علوم پزشکی شیراز، شیراز، ایران
فرزانه واحدی | Farzaneh Vahedi
Assistant Professor, Cellular and Molecular Research Center, Babol University of Medical Sciences, Babol, Iran
استادیار، مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی بابل، بابل، ایران
مرتضی طاهری انگنه | Mortaza Taheri-Anganeh
Assistant Professor, Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
استادیار، مرکز تحقیقات سلولی و مولکولی، پژوهشکده پزشکی سلولی و مولکولی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران
خدیجه احمدی | Khadijeh Ahmadi
Assistant Professor, Infectious and Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
استادیار، مرکز تحقیقات بیماری های عفونی و گرمسیری، دانشگاه علوم پزشکی هرمزگان، بندرعباس، ایران
مهدی علیزاده | Mehdi Alizadeh
PhD in Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
دکتری بیوشیمی بالینی، گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران
سید حسین خاتمی | Seyyed Hossein Khatami
PhD in Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
دکتری بیوشیمی بالینی، گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران
محمد صباغان | Mohamad Sabaghan
Assistant Professor, Department of Basic Sciences, Behbahan Faculty of Medical Sciences, Behbahan, Iran
استادیار، گروه علوم پایه، دانشکده علوم پزشکی بهبهان، بهبهان، ایران
رحمت الله سلطانی | Rahmatollah Soltani
Assistant Professor, Department of Basic Sciences, Behbahan Faculty of Medical Sciences, Behbahan, Iran
استادیار، گروه علوم پایه، دانشکده علوم پزشکی بهبهان، بهبهان، ایران
وحید زارع زاده | Vahid Zarezade
Assistant Professor, Department of Basic Sciences, Behbahan Faculty of Medical Sciences, Behbahan, Iran
استادیار، گروه علوم پایه، دانشکده علوم پزشکی بهبهان، بهبهان، ایران
احمد موحدپور | Ahmad Movahedpour
Assistant Professor, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
استادیار، مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
حسن قاسمی | Hassan Ghasemi
Associate Professor, Department of Basic Sciences, Abadan University of Medical Sciences, Abadan, Iran
دانشیار، گروه علوم پایه، دانشگاه علوم پزشکی آبادان، آبادان، ایران
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