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Iranian Biomedical Journal، جلد ۲۹، شماره ۶، صفحات ۳۹۷-۴۰۴
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Transcriptional Control of PI3K/AKT/mTOR by piRNA-651 in Breast Carcinoma
چکیده انگلیسی مقاله
Background: Breast cancer (BC) is among the most prevalent malignancies in women worldwide, yet early diagnosis is associated with a high survival rate. The proliferation of BC is linked to the overexpression of genes within the PI3K/AKT/mTOR signaling pathway. piR-651 has been reported to be effective in the proliferation and metastasis of BC. This research sought to evaluate the impact of piR-651 inhibition on the PI3K/AKT/mTOR pathway in HUVEC, MCF-7, and MDA-MB-231 cells.
Methods: Anti-piR-651 and non-target sequences were introduced into HUVEC, MCF-7, and MDA-MB-231 BC cells by lipofectamine transfection. After 48 hours, total RNA was extracted, and qRT-PCR assessed the gene expression of PI3K, AKT, and mTOR.
Results: Anti-piR-651 treatment significantly increased PI3K, AKT, and mTOR gene expression in HUVECs (p < 0.001). In contrast, PI3K and mTOR expression decreased in MCF-7 and MDA-MB-231 cells (p < 0.001), while AKT expression remained unchanged in MDA-MB-231 cells (p > 0.05). Correlations between these genes varied by cell type, with significant associations observed at p < 0.05 or p < 0.01, depending on the group.
Conclusion: piR-651 inhibition causes AKT to behave independently of PI3K and mTOR, particularly in MCF-7 cells, suggesting limited gene therapy potential for estrogen receptor-positive BC. Preliminary data indicate that piR-651 inhibition may reduce BC cell proliferation through effects on PI3K and mTOR.
کلیدواژه‌های انگلیسی مقاله Epigenetics, Piwi-interacting RNA, Triple negative breast neoplasms
نویسندگان مقاله | Sena Köstü
Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY


| Baturalp Bakirci
Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY


| Ece Bilge Şimşek
Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY


| Defne Dumanli
Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY


| Ahmetcan Çelik
Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY


| Alara Kaya
Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY


| Damla Kolcuoğlu
Kırklareli University, Faculty of Medicine, Department of Medical Biology, Kırklareli, TURKEY


| Ertuğrul Çolak
Eskişehir Osmangazi University, Faculty of Medicine, Department of Biostatistics, Eskişehir, TURKEY


| Çağrı Öner
Kırklareli University, Faculty of Medicine, Department of Medical Biology, Kırklareli, TURKEY
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-6451-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cancer Biology
نوع مقاله منتشر شده مقاله کامل
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