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Iranian Journal of Parasitology، جلد ۱۱، شماره ۱، صفحات ۸۶-۹۰
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عنوان انگلیسی Microscopic and Molecular detection of Theileria (Babesia) equi infection in equids of Kurdistan Province, Iran
چکیده انگلیسی مقاله Background: Equine piroplasmosis (EP) is the cause of persistent tick-borne infection with no symptoms, but the most important problem of EP is due to the persistent carrier state. Carrier animals to Babesia ( Theileria ) equi (Laveran 1901) and B. caballi (Nuttall, 1910) infestation could be identified by extremely sensitive PCR-based method. The purpose of this study was to identify the causative agents of equine piroplasmosis based on molecular and microscopic assays in equids from Kurdistan Province, Iran. Methods : Thirty one horse and mule blood samples were used with history of liv­ing in Kurdistan Province of Iran. The blood specimens were utilized for T. equi and B. caballi DNA identification by PCR and Giemsa stained smears for micro­scopic observation. Results : The results clearly showed the presence of B. ( Theileria ) equi DNA in 30 of 31 blood samples (96.77%), but the microscopic examination revealed the 3 of 31 positive Babesia like organisms in the red blood cells (9.67%). Conclusion : The obtained results demonstrated the presence of hidden B. ( Theil­eria ) equi infection in horses with previous habitance in Kurdistan Province of Iran. The carrier animals became a main source of infection and can transmit the disease. Therefore, hidden infection might be considered as a health threatening and limit­ing factor in animals used in therapeutic antisera research and production centers.
کلیدواژه‌های انگلیسی مقاله Theileria, Babesia equi, PCR, Iran
نویسندگان مقاله غلامرضا حبیبی | gholamreza habibi


کسری اسماعیل نیا | kasra esmaeilnia


محمد حسن حبل الورید | mohammad hasan hablolvarid


اصغر افشاری | asghar afshari


محسن ضامن | mohsen zamen


صغری بزرگی | soghra bozorgi


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/814
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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