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Iranian Journal of Parasitology، جلد ۱۰، شماره ۴، صفحات ۵۳۰-۵۴۰
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عنوان انگلیسی Comparison of the Proteome Profiling of Iranian isolates of Leish¬mania tropica, L. major and L. infantum by Two- Dimen¬sional Electrophoresis (2-DE) and Mass-spectrometry
چکیده انگلیسی مقاله Background: The mechanisms of virulence and species differences of Leishmania parasites are under the influence of gene expression regulations at posttranscriptional stages. In Iran, L. major and L. tropica are known as principal agents of cutaneous leishmaniasis, while L. infantum causes visceral leishmaniasis. Methods: As a preliminary study, we compared the proteome mapping of the above three Iranian isolates of Leishmania species through the 2-dimension electrophoresis (2-DE), and identified the prominent proteins by Liquid Chromatography (LC) mass spectrometry. Results : We reproducibly detected about 700 protein spots in each species by using the Melanie software. Totally, 264 proteins exhibited significant changes among 3 species. Forty nine protein spots identified in both L. tropica and L. major were similar in position in the gel, whereas only 35 of L. major proteins and 10 of L. tropica proteins were matched with those of L. infantum. Having identified 24 proteins in the three species, we sought to pro­vide possible explanations for their differential expression patterns and discuss their rele­vance to cell biology. Conclusion : The comparison of proteome profiling pattern of the 3 species identified limit up and limit down regulated or absent /present proteins. In addition, the LC-MS data analy­sis showed that most of the protein spots with differential abundance in the 3 species are involved in cell motility and cytoskeleton, cell signaling and vesicular trafficking, intracellu­lar survival / proteolysis, oxidative stress defense, protein synthesis, protein ubiquitina­tion / proteolysis, and stress related proteins. Differentially proteins distributed among the species maybe implicated in host pathogenecity interactions and parasite tropism to cutaneous or visceral tissue macrophages
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نویسندگان مقاله هما حجاران | homa hajjaran
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences tums , tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

میترا محمدی بازرگانی | mitra mohammadi bazargani
agriculture institute, iranian research organization for science and technology, tehran, iran


مهدی محبعلی | mehdi mohebali
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences tums , tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ریچارد burchmore | richard burchmore
institute of infection, immunity and inflammation, college of medical, veterinary and life science, university of glasgow, united kingdom


قاسم حسینی salekdeh | ghasem hosseini salekdeh
agricultural biotechnology research institute of iran, abri , karaj, iran


الهام کاظمی راد | elham kazemi rad
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences tums , tehran, iran and dept. of parasitology, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد رضا خرمی زاده | mohammad reza khoramizadeh
biosensor research center, endocrinology and metabolism molecular-cellular sciences institute, tums, tehran, iran and dept. of medical biotechnology, school of advanced technologies in medicine, tums, tehran, iran
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/651
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زبان مقاله منتشر شده en
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