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Iranian Journal of Parasitology، جلد ۱۰، شماره ۴، صفحات ۵۹۰-۵۹۸
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عنوان انگلیسی Evaluation of Immunogenicity of Cocktail DNA Vaccine Contain¬ing Plasmids Encoding Complete GRA5, SAG1, and ROP2 Antigens of Toxoplasma gondii in BALB/C Mice
چکیده انگلیسی مقاله Background: Severe and fatal complications of toxoplasmosis urge development of effective vaccines against the disease. The current study was performed to evalu­ate cocktail DNA vaccine containing plasmids encoding GRA5, SAG1, and ROP2 genes of Toxoplasma gondii in BALB/c mice in Tarbiat Modares University in 2012. Methods: The plasmids containing complete GRA5, SAG1, and ROP2 genes were mass extracted and then the recombinant plasmids were administered via intramuscu­lar injections according to immunized mice three times with three-week intervals. Then splenocytes were cultured, and proliferation as well as cytokine as­says were carried out. The other mice in each group were inoculated by the parasite and mortality of the mice was evaluated on a daily basis. Results: The results of cytokine assay for INF-γ were higher in the mice that re­ceived the cocktail DNA containing recombinant plasmids. Evaluation of prolifera­tion of splenocytes using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazo­lium bromide) assay indicated induction of cellular response. Measurement of total IgG and the isotypes of IgG1 and IgG2a showed that the cocktail DNA stimulated IgG and IgG2a production in comparison with the control groups ( P < 0.05). Furthermore, the survival rate of mice in the groups that received the cocktail DNA was significantly higher than that in the control groups ( P < 0.05). Conclusion: Administration of the cocktail DNA vaccine led to production of higher levels of IFN-γ, confirmed by secretion of IgG2a, and the immune response was shifted toward Th1. Thus, the cocktail DNA containing the recombinant plas­mids can be an appropriate candidate for immunization against toxoplasmosis.
کلیدواژه‌های انگلیسی مقاله Toxoplasma gondii,Cocktail DNA Vac-cine,pcGRA5,pcSAG1,pcROP2
نویسندگان مقاله رضی ناصری فر | razi naserifar
department of medical parasitology, faculty of medicine, ilam university of medical sciences, ilam, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

فاطمه غفاری فر | fatemeh ghaffarifar
department of parasitology and entomology, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

عبدالحسین دلیمی | abdolhosein dalimi
department of parasitology and entomology, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

زهره شریفی | zohreh sharifi
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

کاووس صلح جو | kavous solhjoo
department of medical microbiology, faculty of medicine, jahrom university of medical sciences, jahrom, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جهرم (Jahrom university of medical sciences)

کامی حسینیان خسروشاهی | kami hosseinian khosroshahi
department of parasitology and entomology, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/660
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