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Iranian Journal of Parasitology، جلد ۱۰، شماره ۳، صفحات ۳۶۶-۳۸۰
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عنوان انگلیسی Comparative Proteomic Profiling of Leishmania tropica: Investiga¬tion of a Case Infected with Simultaneous Cutaneous and Viscero¬tropic Leishmaniasis by 2-Dimentional Electrophoresis and Mass Spectrometry
چکیده انگلیسی مقاله Background: Viscerotropic leishmaniasis caused by Leishmania tropica poses a significant prob­lem in the diagnosis and treatment management. Since differential gene expression is more im­portant in outcome of the infection, we employed proteomic approach to identify potential pro­teins involved in visceralization of L. tropica . Methods: The proteomes profiling of L. tropica isolated from cutaneous and visceral tissues of one host were compared by 2-DE/MS proteomics study. Moreover, the transcript level of some identified proteins was confirmed using real-time RT-PCR. Results: Of the 700 protein spots that were detected reproducibly on each gel, 135 were found to be differentially expressed ( P ≤ 0.05). Most of responsive proteins in visceral isolate changed in less abundant compared to cutaneous isolate. Among differentially expressed proteins, 56 proteins were confidently identified and classified according to the biological process. The larg­est groups consist of proteins involved in carbohydrate metabolism and protein synthesis. Most of the identified proteins, which implicated in energy metabolism, cell signaling and virulence were down-regulated, whereas some proteins that have a role in protein folding, antioxidant defense and proteolysis were up-regulated in visceral form. Moreover, the transcript level of some identified proteins such as co-chaperon was confirmed using real-time RT-PCR. Conclusion: L. tropica probably uses different mechanisms for survival and multiplication in viscera to establish viscerotropic leishmaniasis. The current study provides some clues into the mechanisms underlying the dissemination of L. tropica .
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نویسندگان مقاله هما حجاران | homa hajjaran
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

پریسا موسوی | parisa mousavi
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ریچارد burchmore | richard burchmore
institute of infection, immunity and inflammation, college of medical, veterinary and life science, university of glasgow, united kingdom


مهدی محبعلی | mehdi mohebali
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and research center for endemic parasites of iran, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

میترا محمدی بازرگانی | mitra mohammadi bazargani
agriculture institute, iranian research organization for science and technology, tehran, iran


قاسم حسینی salekdeh | ghasem hosseini salekdeh
agricultural biotechnology research institute of iran, abri , karaj, iran and royan institute for stem cell biology and technology, accer, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

الهام کاظمی راد | elham kazemi rad
dept. of medical parasitology, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

محمد رضا خرمی زاده | mohammad reza khoramizadeh
biosensor research center, endocrinology and metabolism molecular-cellular sciences institute, tehran university of medical sciences, tehran, iran and dept. of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/537
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