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Iranian Journal of Parasitology، جلد ۱۰، شماره ۳، صفحات ۴۴۱-۴۴۷
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عنوان انگلیسی Prevalence and Genetic Characterization of Cryptosporidium Spp. In Diarrheic Children from Gonbad Kavoos City, Iran
چکیده انگلیسی مقاله Background: Cryptosporidium is an intestinal protozean parasite causing water­borne and foodborne outbreaks of diarrheal diseases. The present study was per­formed in order to find prevalence and subtypes of Cryptosporidium among children with diarrhea in Gonbad Kavoos City, Northern Iran. Methods: Diarrheic samples were collected from 547 children. The initial parasitologi­cal diagnosis was made based on detection of oocysts using the modi­fied Ziehl-Neelsen acid-fast staining method. The positive microscopically samples were selected for sequence analysis of partial 60 kDa glycoprotein ( gp60 ) gene. Results: Out of 547 collected samples, 27 (4.94%) were positive for Cryptosporid­ium oocysts. Fifteen from 27 positive samples successfully amplified in PCR. Se­quences analysis of gp60 gene in 15 Cryptosporidium isolates revealed that all of them (100%) were C. parvum . The results showed three subtypes of IIa subtype family (7 cases) including IIaA16G2R1, IIaA17G1R1, IIaA22G3R1 and one subtype of IId subtype family (8 cases). The most common allele was IId A17G1d (53.3%). Conclusion: The predominance of zoonotic subtype families of C. parvum species (IIa, IId) in the present study is in concordance with previous studies in Iran and emphasizes the significance of zoonotic transmission of cryptosporidiosis in the country.
کلیدواژه‌های انگلیسی مقاله Cryptosporidium,Subtypes,Gp60 gene,Children,Iran
نویسندگان مقاله میترا شربت خوری | mitra sharbatkhori
laboratory science research center, golestan university of medical sciences, gorgan, iran and dept. of parasitology and mycology, school of medicine, golestan university of medical sciences, gorgan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)

احسان احسان مجرد | ehsan nazemalhosseini mojarad
gastroenterology and liver diseases research center, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نیلوفر تقی پور | niloofar taghipour
gastroenterology and liver diseases research center, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

عبدل ستار پقه | abdol sattar pagheh
toxoplasmosis research center, mazandaran university of medical sciences, sari, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

فاطمه مسگریان | fatemeh mesgarian
laboratory of gonbad health center, golestan university of medical sciences, gorgan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/310
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