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Iranian Journal of Parasitology، جلد ۱۰، شماره ۳، صفحات ۴۴۸-۴۵۶
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عنوان انگلیسی Characterization of Specific RAPD Markers of Virulence in Tri-chomonas vaginalis Isolates
چکیده انگلیسی مقاله Background: As for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. Specific Random Amplified Polymorphic DNA (RAPD) markers of 490 bp, 720 bp and 460 bp using the primers Tv-5, OPA-6 and OPA-11, respectively, were reported. This was the first description of possible ge­netic virulence markers of the infection by T. vaginalis . The aim of this study was to characterize the specific RAPD markers in order to elucidate their importance on virulence of this illness. Methods: The selected specific RAPD fragments were cloned and sequenced. The obtained sequences were compared by the BLAST algorithm. Results: The nucleotide sequence of the Tv-5 490 RAPD marker exhibited signifi­cant similarity to T. vaginalis hypothetical G3 leucine rich repeat (LRR) family pro­tein (e-value: 6e-14) and Giardia lamblia leucine rich repeat protein 1 virus receptor protein (e-value: 6e-14 and 2e-12) ; however, the OPA-6 720 and OPA-11 460 showed no significant similarity with any coding published sequence. All the evaluated strains showed the presence of the LRR gene. Conclusion: These results demonstrate a possible role of this gene in the viru­lence of T. vaginalis and in the parasite infection with Trichomonas virus as a possible virus receptor. Further analysis of this gene and encoded protein will allow determin­ing the role that they play in the isolates virus susceptible or resistant pheno­types.
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نویسندگان مقاله jorge fraga | jorge fraga
dept. of parasitology, institute of tropical medicine pedro kourí, havana, cuba


lázara rojas | lázara rojas
dept. of parasitology, institute of tropical medicine pedro kourí, havana, cuba


idalia sariego | idalia sariego
dept. of parasitology, institute of tropical medicine pedro kourí, havana, cuba


aymé فرناندز calienes | aymé fernández calienes
dept. of parasitology, institute of tropical medicine pedro kourí, havana, cuba
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/311
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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