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Iranian Journal of Parasitology، جلد ۱۰، شماره ۳، صفحات ۴۶۵-۴۷۲
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عنوان انگلیسی Genotyping Characterization of Toxoplasma gondii in Cattle, Sheep, Goats and Swine from the North of Portugal
چکیده انگلیسی مقاله Background: Epidemiological investigations on Toxoplasma gondii infection have found a significant association between human toxoplasmosis and consumption of raw or undercooked meat. The present study aimed to characterize genotypes of T. gondii in 20 cattle, 40 sheep, 15 goats and 16 pigs from the North of Portugal. Methods: Nested PCR amplified the surface antigen 2 (SAG2) gene. Sequencing analy­sis was performed in order to assess the prevalence of SAG2 type strains (I, II and III). Results: Three and 4 strains of SAG2 type II were identified in heart samples of cattle and sheep, respectively. Three SAG2 type II strains were detected in brain, diaphragm and heart of 3 pigs. Three strains detected in heart samples of 3 goats belonged to SAG2 types I or II; with the same result being observed in heart samples from 2 sheep and in 2 brain and 1 heart samples from 3 pigs. Conclusion: SAG2 type II has been shown for the first time to infect cattle in North of Portugal. In addition, SAG2 type II has also been confirmed as the predominant strain in sheep and pigs in the same region. This is the first molecular report of T. gondii in goats from Portugal.
کلیدواژه‌های انگلیسی مقاله Genotypes,Portugal,Ruminants,Swine,Toxoplasma gon-dii
نویسندگان مقاله آنا patrícia lopes | ana patrícia lopes
department of veterinary sciences, school of agrarian and veterinary sciences, university of trás-os-montes e alto douro utad , vila real, portugal and animal and veterinary research centre cecav , university of trás-os-montes e alto douro utad , vila real, portugal


anabela vilares | anabela vilares
department of infectious diseases, national institute of health, lisbon, portugal


فرانسیسکو neto | francisco neto
department of veterinary sciences, school of agrarian and veterinary sciences, university of trás-os-montes e alto douro utad , vila real, portugal


alcina رودریگویز | alcina rodrigues
veterinary services directorate of north region, veterinary general-directorate, ministry of agriculture, portugal


tânia martins | tânia martins
veterinary services directorate of north region, veterinary general-directorate, ministry of agriculture, portugal


idalina فریرا | idalina ferreira
department of infectious diseases, national institute of health, lisbon, portugal


ماریا joão gargaté | maria joão gargaté
department of infectious diseases, national institute of health, lisbon, portugal


manuela رودریگویز | manuela rodrigues
department of veterinary sciences, school of agrarian and veterinary sciences, university of trás-os-montes e alto douro utad , vila real, portugal and animal and veterinary research centre cecav , university of trás-os-montes e alto douro utad , vila real, portugal


luís cardoso | luís cardoso
department of veterinary sciences, school of agrarian and veterinary sciences, university of trás-os-montes e alto douro utad , vila real, portugal and parasite disease group, instituto de biologia molecular e celular ibmc , universidade do porto, portugal
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/313
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