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Iranian Journal of Parasitology، جلد ۱۰، شماره ۲، صفحات ۱۸۹-۱۹۶
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عنوان انگلیسی Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization.
چکیده انگلیسی مقاله Background : Haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. The present study was carried out to differentiate Haemonchus species from its main hosts in Iran, including sheep, goat and camel. Methods: The identification took place based on the morphometrics of the spic-ules and molecular characters. Two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each ani-mal) at the slaughterhouses from different localities in Iran. Samples were morpho-logically identified according to the spicules’ morphometric measurements. In the section on molecular study, 10 samples of each Haemonchus isolates were genetically examined. A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results. Results: PCR-RFLP profile obtained from the restriction enzyme HPa1 in H. con-tortus and H. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different frag-ments, respectively. The morphological parameters clearly distinguish H. contortus from H. longistipes. Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively. Conclusion: The genotypic results are in agreement with the phenotypic findings of both species.
کلیدواژه‌های انگلیسی مقاله Haemonchus species,ITS2,Morphology,PCR-RFLP
نویسندگان مقاله بهنام مشگی | behnam meshgi
dept. of parasitology, faculty of veterinary medicine, university of tehran, iran center of excellent of ecosystem and ultrastructural changes of helminthes .

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

فاطمه جالوسیان | fatemeh jalousian
dept. of parasitology, faculty of veterinary medicine, university of tehran, iran center of excellent of ecosystem and ultrastructural changes of helminthes .

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

زهرا مسیح | zahra masih
dept. of parasitology, faculty of veterinary medicine, university of tehran, iran center of excellent of ecosystem and ultrastructural changes of helminthes .

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/333
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