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Iranian Journal of Parasitology، جلد ۱۰، شماره ۲، صفحات ۲۱۳-۲۲۹
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عنوان انگلیسی Abnormalities of Endocytosis, Phagocytosis, and Development Process in Dictyostelium Cells That Over-Express Acanthamoeba castellanii Metacaspase Protein.
چکیده انگلیسی مقاله Background : Acanthamoeba castellanii forms a resistant cyst that protects the para-site against the host’s immune response. Acanthamoeba Type-I metacaspase (Acmcp) is a caspase-like protein that has been found to be expressed during the encysta-tions. Dictyostelium discoideum is an organism closely related to Acanthamoeba useful for studying the molecular function of this protozoan caspase-like protein. Methods: The full length of Acmcp and a mutated version of the same gene, which lacks the proline rich N-terminal region (Acmcp-dpr), were cloned into the pDneo2a-GFP vector separately. The pDneo2a-GFP-Acmcp and pDneo2a-GFP-Acmcp-dpr were electro-transfected into wild type D . discoideum cells to create cell lines that over-expressed Acmcp or Acmcp-dpr. Results : Both cell lines that over-expressed Acmcp and Acmcp-dpr showed a sig-nificant increase in the fluid phase internalization and phagocytosis rate compared to the control cells. Additionally, the cells expressing the Acmcp-dpr mutant were unable to initiate early development and failed to aggregate or form fruiting bodies under starvation conditions, whereas Acmcp over-expressing cells showed the op-posite phenomena. Quantitative cell death analysis provided additional support for these findings. Conclusion : Acmcp is involved in the processes of endocytosis and phagocytosis. In addition, the proline rich region in Acmcp is important for cellular development in Dictyostelium . Given its important role in the development process, metacaspase protein is proposed as a candidate drug target against infections caused by A. castel-lanii .
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نویسندگان مقاله entsar صاحب | entsar saheb
dept. of biology, college of sciences, university of baghdad, baghdad, iraq.


وندی trzyna | wendy trzyna
dept. of biological sciences, marshall university, huntington, west virginia, usa.


katherine maringer | katherine maringer
dept. of biology, college of science and mathematics, university of arkansas at little rock, arkansas, usa.


جان بوش | john bush
dept. of biology, college of science and mathematics, university of arkansas at little rock, arkansas, usa.
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/330
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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