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Iranian Journal of Parasitology، جلد ۱۰، شماره ۲، صفحات ۲۵۰-۲۵۷
عنوان فارسی
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عنوان انگلیسی Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.
چکیده انگلیسی مقاله Background : Free-living amoebae such as Acanthamoeba species may act as carriers of Cryptosporidium and Toxoplasma oocysts, thus, may play an important role in the water-borne transmission of these parasites. In the present study, a loop mediated isothermal amplification (LAMP) method for detection of Toxoplasma and a PCR assay were developed for investigation of Acanthamoeba in environmental water samples. Methods: A total of 34 samples were collected from the surface water in Guilan Province. Water samples were filtrated with membrane filters and followed by DNA extraction. PCR and LAMP methods used for detection of the protozoan parasites Acanthamoeba and Toxoplasma respectively . Results : Totally 30 and 2 of 34 samples were positive for Acanthamoeba and Toxoplasma oocysts respectively. Two samples were positive for both investigated parasites. Conclusion: The investigated water supplies, are contaminated by Toxoplasma and Acanthamoeba (oo)cystes. Acanthamoeba may play an important role in water-borne transmission of Toxoplasma in the study area. For the first time in Iran, protocol of LAMP method was used effectively for the detection of Toxoplasma in surface water samples in Iran.
کلیدواژه‌های انگلیسی مقاله Acanthamoeba,Iran,LAMP,PCR,Toxoplasma
نویسندگان مقاله محمد رضا محمودی | mohammad reza mahmoudi
dept. of medical microbiology and parasitology, school of medicine, guilan university of medical sciences, rasht, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گیلان (Guilan university of medical sciences)

بهرام کاظمی | bahram kazemi
dept. of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

علی حقیقی | ali haghighi
cellular and biology research centre, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

panagiotis karanis | panagiotis karanis
center for biomedicine and infectious diseases cbid , qinghai academy for animal sciences and veterinary medicine, qinghai university, xining, china.
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/326
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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