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Iranian Journal of Parasitology، جلد ۹، شماره ۴، صفحات ۴۴۵-۴۵۱
عنوان فارسی
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عنوان انگلیسی Comparison of three staining methods for the detection of intestinal microspora spp.
چکیده انگلیسی مقاله Background : This study aimed to compare three staining methods including: Calcofluor white, Chromotrope and Quick Hot Gram chromotrope used in diagnosis of intestinal microsporidial spores . Methods : One hundred and seventy five stool specimens were collected from patients referred to Laboratory of Intestinal Protozoology at the School of Public Health, Tehran university of Medical Sciences during 2012-2013. All of specimens were evaluated by nested PCR. The formalin–fixed stool samples were prepared from each specimen and dried at room temperature for 10 min, followed by 10 min methanol fixation. All the collected stoolsamples were evaluated blindly by calcofluor white, Chromotrope and Quick Hot Gram chromotrope staining methods separately. Results : Microsporidial spores were recognized using Chromotrope, Quick Hot Gram chromotrope and Calcofluor white , in16 of 18 (88.8%), 17 of 18 (94.4%) and 18 of18(100%) samples that were positive by nested PCR respectively . Regarding 14 stool samples that were negative by nested PCR, 14 cases were negative bychromotrope and Quick hot Gram chromotrope and 13 samples were negative by Calcofluor white. One discordant sample interpreted as false positive. Conclusion: Calcofluor white staining had the best performance for the detection of intestinal Microsprora spores and can be used as initial screen test for the detection of intestinal Microspora spp
کلیدواژه‌های انگلیسی مقاله Calcofluor,Chromotrope,Gram stain,Microspora,Staining
نویسندگان مقاله خدیجه خانعلی ها | khadijeh khanaliha
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حامد میرجلالی | hamed mirjalali
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی محبعلی | mehdi mohebali
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فاطمه طریقی | fatemeh tarighi
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مصطفی رضاییان | mostafa rezaeian
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/379
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