این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۹، شماره ۴، صفحات ۴۸۲-۴۹۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Isolation of Small Number of Cryptosporidium parvum Oocyst Using Immunochromatography.
چکیده انگلیسی مقاله Background: Cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. The organism can be transmitted through water. Since small number of C. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of C. parvum oocyst in samples with limited number of oocysts. Methods: Antibody was prepared against whole antigen from C. parvum oocysts, the achieved Ab bound to the sepharose 4B and used for the isolation of oocysts. Antibody against P23 bound to the sepharose 4B, used also for the isolation of C. parvum oocyst. In comparison to these both methods, 2 traditional methods (Salt floatation and 55% sucrose floatation) were also performed. Results: Both chromatography methods could bind oocysts with capacity depends on the column size. The isolated oocysts were free of bacteria. Our results showed that the traditional methods are useful for the isolation of oocysts from feces, in its smear stained with ziehl-nelsen, at least 3 oocyts are detectable in each microscopic field under 1000 X magnification. In contrast to the chromatography methods, the bacterial contamination was always observed in oocysts isolated with traditional methods. Conclusion: Immunochromatography could be used for the successful isolation of C. parvum oocysts from the samples containing limited number of oocysts.
کلیدواژه‌های انگلیسی مقاله Chromatography,Cryptosporidium parvum,Flotation,Oocyst
نویسندگان مقاله الهه ابراهیم زاده | elahe ebrahimzade
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

پرویز شایان | parviz shayan
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

زینب اصغری | zeinab asghari
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

صدیقه جعفری | sedighe jafari
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

زهرا امیدیان | zahra omidian
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/374
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات