| چکیده انگلیسی مقاله |
Background: Cytokines play a fundamental role in the regulation of immune responses in remission and/or relapsing of leishmaniasis. Therefore, immunotherapy for the treatment of canine visceral leishmaniasis (CVL) has represented a principle approach in control of the infection. The present research aimed to evaluating the immunotherapeutic potential of a novel herbal immunomodulator drug (IMOD) on CVL. Methods: Twelve mongrel dogs were intravenously infected with Iranian strain of L. infantum and randomly divided into three groups; 1: negative control (non-infected), 2: immunotherapy with IMOD and 3: positive control (non-treated). Cell proliferation and Th1-/Th2-type cytokines were measured in peripheral blood mononuclear cell (PBMC) by cell proliferation kit I (MTT) and enzyme-linked immunospot (ELISpot) assays, respectively. Results : At the 60 days follow-up assessment, no adverse effects were observed in treated interventional group. Cellular proliferation assay indicated that PBMCs of IMOD group had higher stimulation index (SI) than positive control group ( p < 0.05). Enhancement of CD4+ T cells such as IL-2, IL-4 & IL-10 were detected in negative control group due to in vitro IMOD stimulation 30 days post-treatment. In accordance to decreasing trends of Th1 & Th2 cytokines in positive control group, the mean number of IFN-γ, IL-2, IL-4 and IL-10 spot forming cells (SFCs) down regulated for IMOD group during the study. Conclusion : These data indicate that IMOD had immunomodulatory potential but is not sufficient for total parasitic cure due to balance of Th1/Th2 cytokines. This is a preliminary study and we propose to undertake a series of experiments to evaluate the CVL due to in vitro modulatory effects of IMOD. |
| نویسندگان مقاله |
عبدالعلی ملماسی | abdolali malmasi
department of internal medicine, faculty of veterinary medicine, university of tehran, tehran, iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
بیژن ضیایی اردستانی | bijan ziaie ardestani
department of internal medicine, faculty of veterinary medicine, university of tehran, tehran, iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
سعید الحق | saeed bayanolhagh
iranian research center for hiv aids ircha , tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
مهدی محبعلی | mehdi mohebali
department of parasitology, faculty of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
حمیدرضا خرم خورشید | hamidreza khorram khorshid
genetic research center, university of social welfare and rehabilitation sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم بهزیستی و توانبخشی (University of social welfare and rehabilitation sciences)
پریسا صدرپور | parisa sadrpour
iranian research center for hiv aids ircha , tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
نگین حسینی روزبهانی | negin hosseini rouzbahani
iranian research center for hiv aids ircha , tehran university of medical sciences, tehran, iran and department of immunology, faculty of medicine, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
بهناز آخوندی | behnaz akhoundi
department of parasitology, faculty of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
سولماز ناصرلی | soulmaz naserli
department of pathobiology, faculty of veterinary medicine, university of tehran, tehran, iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
شادی ضیایی | shadi ziaie
department of clinical pharmacy, faculty of pharmacy, shahid-beheshti university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
احسان مصطفوی | ehsan mostafavi
department of epidemiology, pasteur institute of iran, tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
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