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Iranian Journal of Parasitology، جلد ۹، شماره ۳، صفحات ۳۲۹-۳۳۵
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عنوان انگلیسی Genetic Identification of Trichomonas vaginalis by Using the Actin Gene and Molecular Based Methods.
چکیده انگلیسی مقاله Background: Trichomonas vaginalis is the agent of urogenital tract infection that causes human trichomoniasis with some serious health complications. More understanding about genetic features of the parasite can be helpful in the study of the pathogenesis, drug susceptibility and epidemiology of the infection. For this end,we conducted analysis of the actin gene of T. vaginalis by applying the PCR-SSCP(PCR-Single Stranded Conformational Polymorphism) and nucleotide sequencingmethod. Methods: Fifty T. vaginalis samples were collected from 950 women attending gynecology clinics in two cities of Iran, Hamadan and Tehran, from November 2010 to July 2011. After axenisation of isolates, all samples subjected to PCR-SSCP and nucleotide sequencing. Results: According to the SSCP banding patterns and nucleotide sequencing, seven sequence types were detected among the isolates. Alignment of the nucleotide sequences showed five polymorphic sites in the different strain types. Amino acid substitution was not observed in the nucleotide sequence translation of the all sequences. Conclusion: The actin gene analysis represents genetic diversity of T. vaginalis and it suggests that various strains can be responsible for clinically different trichomoniasis in infected individuals. It is expected that further studies will be conductedto increase our knowledge about relationship between the actin gene polymorphism and different biological behavior of the parasite.
کلیدواژه‌های انگلیسی مقاله Actin gene,SSCP,Trichomonas vaginalis
نویسندگان مقاله محمد متینی | mohammad matini
dept. of medical parasitology and mycology, school of medicine, hamadan university of medical sciences, hamadan, iran and dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

ساسان رضایی | sassan rezaie
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی محبعلی | mahdi mohebali
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

امیر حسین مقصود | amir hossein maghsood
dept. of medical parasitology and mycology, school of medicine, hamadan university of medical sciences, hamadan, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

صغری ربیعی | soghra rabiee
dept. of obstetrics and gynecology, fatemieh women hospital, school of medicine, hamadan university of medical sciences, hamadan, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

محمد فلاح | mohammad fallah
dept. of medical parasitology and mycology, school of medicine, hamadan university of medical sciences, hamadan, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

مصطفی رضاییان | mostafa rezaeian
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/395
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