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Iranian Journal of Parasitology، جلد ۹، شماره ۳، صفحات ۳۵۸-۳۶۴
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عنوان انگلیسی PCR-RFLP Analysis of 28 SrDNA for Specification of Fasciola gigantica (Cobbold, 1855) in the Infected Lymnaea auricularia (Linnaeus, 1785) Snails from Northwestern Iran.
چکیده انگلیسی مقاله Background: Fasciolosis in livestock is a crucial concern in the globe, mainly due to its impact on human health. The aim of this study was to determine the rate of infection with Fasciola gigantica (Cobbold, 1855) larvae in the field-collected snails of Lymnaea auricularia (Linnaeus, 1785) from northwestern Iran using a molecular approach. Methods : A total of 6,759 pond snails were collected from 28 freshwater bodies in West Azarbaijan. PCR was performed to amplify a 618-bp fragment of the nuclear 28 SrRNA gene of Fasciola . The PCR products were digested by Ava II restriction enzyme to create restriction fragment length polymorphism (RFLP) patterns specific for the detection of F. gigantica . Results : Of the total collected snails 496 (7.34 %) were L. auricularia , among which 4.64% (23 out of 496) were infected with a Fasciola species according to the PCR analysis. Only 2.22% (11 out of 496) of the infected snails were from the mountainous areas. The highest Fasciola infection rate recorded in the snails of a single site was 1.81% (9 out of 496 snails). Based on the RFLP pattern, F. gigantica accounted for 2.42% of the infection rates in the study sites. Conclusion : Application of PCR-RFLP was proven to be a useful approach for valid and robust detection of the infection with F. gigantica in its intermediate host snails . These findings may therefore be applicable for establishment of the controlprograms against dissemination of the infection in different regions.
کلیدواژه‌های انگلیسی مقاله 28SrDNA,Fasciola gigantica,Iran,Lymnaea auricularia
نویسندگان مقاله محمد یخچالی | mohammad yakhchali
dept. of pathobiology, parasitology division, faculty of veterinary medicine, urmia university, urmia, iran.

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

رضا ملک زاده viayeh | reza malekzadeh viayeh
artemia and aquatic animals research institute, urmia university, urmia, iran.

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

عباس ایمانی باران | abass imani baran
dept. of pathobiology, parasitology division, faculty of veterinary medicine, tabriz university, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/391
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