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Iranian Journal of Parasitology، جلد ۹، شماره ۳، صفحات ۳۷۴-۳۸۱
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عنوان انگلیسی Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica.
چکیده انگلیسی مقاله Background: The study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of Fasciola gigantica experimentally infected rabbits. Methods: Copro Hyper Immune Serum (HIS) and Excretory-Secretory Hyper Immune Serum (ES HIS) antigens were used in a sandwich ELISA for the detection of F. gigantica antigens in feces of 12 rabbits experimentally infected with different doses of F. gigantica encysted metacercariae (EMC) (10, 25 and 30 EMC). The relation between time of appearance of coproantigens in feces and anti- Fasciola antibodies in serum was evaluated. Results: The earliest diagnostic coproantigen was recorded at 21 st , 25 th and 28 th day post-infection (p.i.) in groups of rabbits infected with 30, 25 and 10 F. gigantica EMC respectively. Both HIS and ES HIS were able to detect coproantigens in feces of rabbits infected with 30 EMC at day 21 p.i. The appearance of F. gigantica coproantigens in feces of infected rabbits was concurrent to the appearance of anti- Fasciola antibodies in blood (3 rd week p.i.). However, coproantigen has specific ability for direct assessment of active infection with minimal cross-reaction with other heterologous parasitic infections. Conclusion: The findings hold promise for a more accurate diagnostic technique in the near future for suspected Fasciola infection.
کلیدواژه‌های انگلیسی مقاله Antibodies,Coproantigen,Diagnosis,Fasciola
نویسندگان مقاله سعید ابراهیم احمد | said ibrahim shalaby
department of complementary medicine, medical division, national research centre, cairo, egypt.


محمد el bahy | mohammad el bahy
department of parasitology, faculty of veterinary medicine, cairo university, cairo, egypt.


سهمی ابراهیم aboulella احمد | samy ibrahim aboulella shalaby
department of reproduction, veterinary division, national research centre, cairo, egypt.


حاتم احمد | hatem shalaby
department of parasitology, veterinary division, national research centre, cairo, egypt.


نیلما گوپتا | neelima gupta
department of animal science, mjp rohilkhand university, bareilly, up, india.


dileep کومار گوپتا | dileep kumar gupta
department of zoology, bareilly college, bareilly, up, india.
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/389
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