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Iranian Journal of Parasitology، جلد ۹، شماره ۳، صفحات ۳۹۴-۴۰۱
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عنوان انگلیسی Isolation, Identification, and Pathogenicity of Neospora caninum China Yanbian strain.
چکیده انگلیسی مقاله Background: The aim of the study was to provide a point of reference to study the Neospora caninum infections in China. Methods: Genome DNA was extracted from the brains of aborted fetuses and specific PCR was performed with N. caninum Nc5-targeted specific primers. Fetal bovine brain tissues were homogenized and continuously cultured in Vero cells with double antibodies. The medium was replaced at 2-d intervals and the state of cells was observed. Results: A 608 bp Nc5 gene band was detected by PCR amplification. After sequencing,the sequence of the sample shared 99.5% homology with GenBank(AF061249). Brain homogenates were continuously cultured in Vero cells for 34 d and N. caninum was found. The results of IFAT and Nc5 gene-based PCR detection were N. caninum -positive, and the parasite was tentatively named N. caninum China Yanbian strain. BABL/c mice were inoculated with the separated parasites and showed clinical symptoms of ataxia and limb paralysis after 12 d. Only 3 mice survived.The blood of dying mice and the hearts, livers, spleens, lungs, kidneys, andbrains of dead mice were collected aseptically. The Nc5 gene-based PCR showed that N. caninum may exist in brains, livers, and spleen. Based on immunohistochemical observations, we showed that N. caninum tachyzoites existed in the brains and livers. Conclusion: We have successfully isolated bovine-specific N. caninum strain from brain tissues of aborted cattle in the China Yanbian region. This isolated strain has a strong infectious ability towards BABL/c mice.
کلیدواژه‌های انگلیسی مقاله Bovine,China,Isolation,Neospora caninum
نویسندگان مقاله li جون جیا | li jun jia
laboratory of veterinary microbiology, department of veterinary medicine, yanbian university, yanji, jilin, china.


shou fa زانگ | shou fa zhang
laboratory of veterinary microbiology, department of veterinary medicine, yanbian university, yanji, jilin, china.


ming ming liu | ming ming liu
laboratory of veterinary microbiology, department of veterinary medicine, yanbian university, yanji, jilin, china.


نیان chao qian | nian chao qian
laboratory of veterinary microbiology, department of veterinary medicine, yanbian university, yanji, jilin, china.


huan ping گیو | huan ping guo
laboratory of veterinary microbiology, department of veterinary medicine, yanbian university, yanji, jilin, china.
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/387
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