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Iranian Journal of Parasitology، جلد ۹، شماره ۳، صفحات ۴۳۵-۴۴۰
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عنوان انگلیسی Molecular and parasitological study of cryptosporidium isolates from cattle in ilam, west of iran.
چکیده انگلیسی مقاله Background: Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. Methods : Fecal samples of 217 cattle were collected fresh and directly from the rectum of cattle. All of the samples were examined by microscopic observation after staining with modified Ziehl-Neelsen (MZN). Genomic DNA extracted by using EURx DNA kit. A Nested PCR-RFLP protocol amplifying 825 bp fragment of 18s rRNA gene conducted to differentiate species and genotyping of the isolates using Ssp I and Vsp I as restriction enzymes. Results : The prevalence of Cryptosporidium infection in cattle using both methods is 3.68%. Most of the positive cattle were calves under six months. Species diagnosis carried out by digesting the secondary PCR product with Ssp I that C. parvum generated 3 visible bands of 448, 247 and 106 bp and digested by Vsp I restriction enzyme generated 2 visible bands of 628 and 104bp. In this investigation all of the positive samples were Cryptosporidium parvum. Conclusion : C. parvum (bovine genotype) detected in all positive cattle samples in Ilam, west of Iran. The results of the present study can help for public health care systems to prevention and management of cryptosporidiosis in cattle and the assessment of cattle cryptosporidiosis as a reservoir for the human infection.
کلیدواژه‌های انگلیسی مقاله Cattle,Cryptosporidium,Iran,PCR-RFLP
نویسندگان مقاله محمود محامی اسکویی | mahmoud mahami oskouei
department of parasitology, faculty of medicine, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

اسماعیل فلاح | esmaeil fallah
department of parasitology, faculty of medicine, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

محمود احمدی | mahmoud ahmadi
international branch of tabriz university of medical sciences aras , tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

عبدالرسول صفاییان | abdolrasoul safaiyan
department of biostatistics, faculty of health, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

سالار بختیاری | salar bakhtiyari
department of clinical biochemistry, faculty of medicine, ilam university of medical sciences, ilam, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

رضی ناصری فر | razi naserifar
department of parasitology, faculty of health, ilam university of medical sciences, ilam, iran and department of parasitology, faculty of paramedicine, ilam university of medical sciences, ilam, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

مجید دوستی | majid dousti
department of parasitology, faculty of paramedicine, ilam university of medical sciences, ilam, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/381
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