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Iranian Journal of Parasitology، جلد ۹، شماره ۲، صفحات ۱۴۹-۱۵۴
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عنوان انگلیسی Emerging Intestinal Microsporidia Infection in HIV(+)/AIDS Patients in Iran: Microscopic and Molecular Detection.
چکیده انگلیسی مقاله Background: Species of Microsporidia have been known as opportunistic obli-gate intracellular parasites particularly in immunocompromised patients. Enterocyto-zoon bieneusi is one of most prevalent intestinal microsporida parasites in HIV + /AIDS patients. In this study, intestinal microsporidia infection was deter-mined in HIV + /AIDS patients using microscopic and molecular methods. Methods: Stool samples were collected from HIV + /AIDS patients during 12 months. All of the stool specimens washed with PBS (pH: 7.5). Slim slides were prepared from each sample and were examined using light microscope with 1000X magnification. DNA extraction carried out in microscopic positive samples. DNA amplification and genus/species identification also performed by Nested-PCR and sequencing techniques. Results: From 81 stool samples, 25 were infected with microsporidia species and E. bieneusi were identified in all of positive samples. No Encephalitozoon spp . was identified in 81 collected samples using specific primers. Conclusion: E. bieneusi is the most prevalent intestinal microsporidia in immuno-compromised patients of Iran. On the other hand, Nested-PCR using specific pri-mers for ssu rRNA gene is an appropriate molecular method for identification of E. bieneusi .
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نویسندگان مقاله حامد میرجلالی | hamed mirjalali
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی محبعلی | mehdi mohebali
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حسین میرهندی | hossein mirhendi
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

رشید غلامی | rashid gholami
dept. of infectious diseases, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حسین کشاورز | hossein keshavarz
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

احمد رضا معمار | ahmad reza meamar
dept. of parasitology and mycology, school of medicine, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

مصطفی رضاییان | mostafa rezaeian
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/419
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