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Iranian Journal of Parasitology، جلد ۹، شماره ۲، صفحات ۲۰۲-۲۰۸
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عنوان انگلیسی Triclabendazole (Anthelmintic Drug) Effects on the Excretory- Secretory Proteome of Fasciola hepatica in Two Dimension Electrophoresis Gel.
چکیده انگلیسی مقاله Background: The aim of this study was to evaluate the protein spots of excre-tory - secretory products of Fasciola hepatica using two dimension electrophoresis method in the presence and absence of triclabendazole drug which can be consid-ered to detect the target protein of the drug. Methods: F. hepatica parasites were collected from infected cattle livers, divided in two groups and cultivated in RPMI 1640 medium. First group was treated with triclabendazole (TCBZ) and second group considered as control. The excretory-secretory (ES) products of each group were separated and total protein deter-mined by Bradford method. To provide proteome spots, the ES proteins were precipitated and two dimension electrophoresis (2-DE) gel prepared. Protein amounts of two groups were compared using the statistical t -test and protein spots from 2-DE in test and control groups were also statistically analyzed. The protein spots of gels were identified by using protein database. Results: The t-test showed a significant increase of total proteins in treated group ( P < 0.5). The protein spots count in the control group was less than test group however statistically not significant (p>0.05). Cathepsin L- protein (MW 36.7 pH 5.34), 14-3-3 epsilon 2 isoform (MW 28.2 pH 5.36), Cathepsin L1D (MW 36.5 pH 5.8) and Cathepsin L1D (MW 36.6 pH 6.26) were identified in test group. Conclusion: It seems that, these results can be considered to determine the pro-teins which the drug acts as a target on them.
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نویسندگان مقاله اشکان فریدی | ashkan faridi
dept. of parasitology and mycology, school of public health, tehran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

علی فرحناک | ali farahnak
dept. of parasitology and mycology, school of public health, tehran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

تقی گل محمدی | taghi golmohammadi
dept. of biochemistry, school of medicine, tehran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمدرضا اشراقیان | mohammadreza eshraghian
dept. of epidemiology and biostatistics, school of public health, tehran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

یوسف شریفی | yosef sharifi
dept. of parasitology and mycology, school of public health, tehran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمدباقر مولایی راد | mohammadbagher molaei rad
dept. of parasitology and mycology, school of public health, tehran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/412
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