این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۹، شماره ۲، صفحات ۲۱۸-۲۲۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Improved serodiagnosis of hydatid cyst disease using gold nanoparticle labeled antigen B in naturally infected sheep.
چکیده انگلیسی مقاله Background: Cystic echinococcosis caused by the metacestode of Echinococcus granulosus is a major problem in both humans and domestic animals health. There-fore, a standardized and approachable diagnostic tool (rapid tests) for the serodiag-nosis of cystic echinococcosis (CE) is still needed. Methods: In the present work, antigen B labeled with gold nanoparticles was used to detect antibodies against hydatid cyst disease. The prepared antigen B was ana-lyzed by SDS-PAGE. Tetra chloroauric acid (HAuCl4) was used to produce colloi-dal gold and antigen B labeled by gold nanoparticles, then it was tested by using rabbits antisera and sera from naturally infected sheep. The labeled antigen B was evaluated using Dot-immunogold staining (Dot-IGS) method. Results: Electrophoretic pattern of hydatid cyst fluid showed the quality of bands in the condensed fluid is better than crude fluid. SDS-PAGE analysis cyst fluid and antigen B revealed three specific protein bands that were detected at molecular weights of 24, 30 and 40 kDa that all are the subunits of antigen B. Evaluation of antigen B labeled by gold nanoparticles by using Dot-IGS technique showed 1/1 and 1/50 dilutions in comparison with another has the best immunoreaction. In this method, nanoparticles produced a typical purple color, when they binded to the strip at the site of immunoreaction. Conclusion: Therefore, using gold nanoparticles is a good candidate for detection of helminthiasis, also as selective tools of early detection, simple and cost-effective, regardless of specific skills and equipment with optimal durability.
کلیدواژه‌های انگلیسی مقاله Antigen B,Diagnosis,Hydatid cyst,Nanoparticle
نویسندگان مقاله زهرا جهانی | zahra jahani
department of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

بهنام مشگی | behnam meshgi
department of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

معصومه رجبی bzl | masomeh rajabi bzl
dept. of medical biochemistry, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

فاطمه جالوسیان | fatemeh jalousian
department of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

سجاد هاشمی نسب | sajad hasheminasab
department of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/410
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات