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Iranian Journal of Parasitology، جلد ۹، شماره ۲، صفحات ۲۲۶-۲۳۲
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عنوان انگلیسی Cryptosporidium infection in patients with gastroenteritis in sari, iran.
چکیده انگلیسی مقاله Background: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore,the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted to hospitals of Mazandaran University of Medical Sciences by parasitological and molecular methods in Sari, Iran. Methods : Stool samples were collected from 348 patients with gastroenteritis admitted to the hospitals of Medical University in the Sari and Ghaemshahr cities in Mazandaran Province, Northern Iran in 2010-2011. Oocysts of Cryptosporidium identified using Formalin-Ether concentration method and stained by Aacid-fast staining (AFS)and Auramine phenol fluorescence (APF). Genomic DAN extracted from microscopically positive samples and nested PCR -RFLP by using SSU rRNA that identifies of the species of cryptosporidium . Results : In 348 patients with gastroenteritis, the most clinical symptoms were diarrhea, nausea, vomiting, dehydration, fever and weight loss. 2.3% (8 cases) of diarrheal samples tested by both microscopy and molecular methods were positive for the presence of cryptosporidium. Nested PCR products yielded unique bands of 846 bp, correspond to cryptosporidium. Species diagnosis carried out by digesting the secondary PCR product with Ssp I restriction enzyme, which noted 3 clearly bands of 449, 254, and 108 bp correspond to Cryptosporidium spp. Conclusion : The results of present study on Cryptosporidium spp. in this area can make a background data for control programs and further molecular analyses. Thus, further work needs to determine the origin of Cryptosporidium species in this area.
کلیدواژه‌های انگلیسی مقاله Cryptosporidium spp,Gastroenteritis Patients,Nested PCR
نویسندگان مقاله شیرزاد غلامی | shirzad gholami
molecular and cell biology research center , mazandaran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

مجید خان محمدی | majid khanmohammadi
department of laboratory sciences, marand branch, islamic azad university, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

احسان احمدپور | ehsan ahmadpour
molecular and cell biology research center , mazandaran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

عبدل ستار پقه | abdol sattar pagheh
molecular and cell biology research center , mazandaran university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

سارا خادم نخجیری | sara khadem nakhjiri
gorgan faculty of medicine, golestan university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)

حمیدرضا ramazannipour | hamidreza ramazannipour
faculty of basic sciences, university of mazandaran, iran.

سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

عباس شهبازی | abbas shahbazi
tabriz research center of infectious and tropical diseases, tabriz university of medical sciences, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/409
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