| چکیده انگلیسی مقاله |
Background: Cutaneous leishmaniasis (CL) is endemic in Southeastern Anatolia, mainly in Sanliurfa and Hatay provinces, and the causative agents are mostly Leishmania tropica and less frequently L. infantum . Here, we report the first MAL-DI-TOF analyses of Leishmania promastigotes obtained from the cultures of two CL cases from Osmaniye and Hatay provinces who were initially diagnosed by microscopy, culture and identified as L. infantum with Real-Time PCR (RT-PCR). Methods: Samples obtained from the skin lesions of patients were initially stained with Giemsa and cultivated in NNN medium. Examination of the smears and cultures revealed Leishmania amastigotes and promastigotes, respectively. The promastigotes (MHOM/TR/2012/CBU15 and MHOM/TR/2012/MK05) ob-tained from the cultures of both patients were used for RT-PCR targeting the ITS-1 region in the SSU of rRNA. The reference strains of four Leishmania spe-cies ( L. infantum, L. donovani, L. tropica and L. major ) were initially assessed with MALDI-TOF and their data were added to MALDI-TOF Biotyper Library. Results: Both RT-PCR and MALDI-TOF analyses indicated that the causative agent in both patient samples was L. infantum . Conclusion: Despite disadvantages such as requirement of culture fluid with nothing but promastigotes and high cost, MALDI-TOF analysis may be a fast, sensitive and specific diagnostic tool in especially large-scale research studies, where the cost declines, relatively. |
| نویسندگان مقاله |
gülnaz culha | gülnaz culha
department of microbiology and clinical microbiology, school of medicine, mustafa kemal university, hatay, turkey.
isin akyar | isin akyar
department of medical microbiology, school of medicine, acibadem university, istanbul, turkey.
fadile yildiz zeyrek | fadile yildiz zeyrek
department of microbiology and clinical microbiology, school of medicine, harran university, sanliurfa, turkey.
özgür kurt | özgür kurt
department of medical microbiology, school of medicine, acibadem university, istanbul, turkey.
cumhur gündüz | cumhur gündüz
department of medical biology, school of medicine, ege university, izmir, turkey.
seray özensoy töz | seray özensoy töz
department of medical parasitology, school of medicine, ege university, izmir, turkey.
ipek östan | ipek östan
vocational school of health sciences, celal bayar university, manisa, turkey.
ابراهیم cavus | ibrahim cavus
department of medical parasitology, school of medicine, celal bayar university, manisa, turkey.
burcu gülkan | burcu gülkan
department of microbiology and clinical microbiology, school of medicine, mustafa kemal university, hatay, turkey.
tanil kocagöz | tanil kocagöz
department of medical microbiology, school of medicine, acibadem university, istanbul, turkey.
یوسف özbel | yusuf özbel
department of medical parasitology, school of medicine, ege university, izmir, turkey.
آحمت özbilgin | ahmet özbilgin
department of medical parasitology, school of medicine, celal bayar university, manisa, turkey.
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