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Iranian Journal of Parasitology، جلد ۹، شماره ۱، صفحات ۲۰-۲۷
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عنوان انگلیسی Genotyping of Echinococcus granulosus Isolates from Human Clinical Samples Based on Sequencing of Mitochondrial Genes in Iran, Tehran.
چکیده انگلیسی مقاله Background: The present study was aimed to investigate molecular diversity of Echino-coccus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in Iran . Methods: Forty seven human hydatid cysts were collected through surgery from two hospitals in Tehran during 2010-2012. To determine the fertility of protoscoleces, the cyst fluids were subjected to morphological microscopic examinations. Protoscoleces were removed from each cyst and their total genomic DNAs were extracted. PCR was performed to amplify fragments of 450 and 400 base pair (bp) for cox1 and nad1 genes, respectively. Genotype diversity and sequence variation of the strains were studied by bioinformatics software and in comparison with those mtDNA sequences already de-posited in GenBank. Results: Sixteen, (53.3%), 13 (43.3%), and 1 (3.3%) samples were related to lung, liver, and spleen, respectively. The remained 17 unfertile samples were excluded from the study. From the 29 isolates, 86.7% (n=26) and 10% (n=3) were related to G1, and G3 genotypes, respectively. The sole isolate with G6 genotype was obtained from lung sample. Analysis of concatenated sequences of cox1 + nad1 indicated the presence of 11 haplotypes among our strains that were related to genotypes G1 (n=9), G3 (n=1) and G6 (n=1). Conclusion: In consistent to other reports from Iran, genotypes G1, G3, and G6 were observed in our human isolates. The rate of G3 genotype was however higher than other studies implying that human can be considered as a new appropriate host for G3 genotype. Further studies with more sample size from different geographic areas of Iran are needed for E. granulosus mapping.
کلیدواژه‌های انگلیسی مقاله Echinococcus granulosus,Genotyping,Iran,cox1,nad1
نویسندگان مقاله بهرام نیک منش | bahram nikmanesh
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حسین میرهندی | hossein mirhendi
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

زهره قلاوند | zohreh ghalavand
iranian tissue bank amp;amp; research center, tehran university of medical science, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

مسعود آل بویه | masoud alebouyeh
gastroenterology and liver diseases research center, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

میترا شربت خوری | mitra sharbatkhori
department of parasitology, school of medicine, golestan university of medical science, gorgan, iran.

سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

eshratbeigom کیا | eshratbeigom kia
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی محبعلی | mehdi mohebali
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مریم اقبالی | maryam eghbali
children medical center hospital, tehran university of medical science, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

محمد باقر رکنی | mohammad bagher rokni
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/437
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