این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۹، شماره ۱، صفحات ۷۰-۷۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Molecular Identification of Giardia duodenalis Isolates from Fars Province, Iran.
چکیده انگلیسی مقاله Background: Giardia duodenalis is one of the most common human intestinal pro-tozoan parasites worldwide and is endemic throughout the world with a vast range of mammalian hosts. The present study aimed to identify the prevalence of G. duo-denalis isolates and determine the most common of its assemblages in the patients referring to health centers and hospitals in Fars province, Iran that will be subjected to further molecular investigation. Methods: We collected 1000 human fecal samples from health centers and hospi-tals in Shiraz, Iran in a one year period from September 2009 to August 2010. Mi-croscopic examination for the presence of G. duodenalis cysts and trophozoites was performed by direct wet mount before and after the concentration techniques. Ex-traction of DNA was performed by Phenol-Chloroform-Isoamylalcohol (PCI). G. duodenalis -positive specimens were analyzed by PCR. A fragment of the SSU-rDNA (292 bp) gene was amplified by PCR using the forward primer RH11 and the re-verse primer RH4. Genotyping was performed using sequence analysis of G. duode-nalis glutamate dehydrogenase gene using primers GDHeF, GDHiF, and GDHiR. Results : The prevalence of Giardia infection was 10.7% (107/1000) examined based on microscopic examination. PCR identified 80% (40/50) of the samples as positive for G. duodenalis based on SSU-rDNA amplification on sucrose gradient samples. Besides, genotyping results indicated 32 isolates (80%) as assemblage AII and 8 isolates (20%) as assemblage BIII and BIV based on the DNA sequence analysis of the glutamate dehydrogenase locus of G. duodenalis . Conclusion : The findings of this study emphasize that Iran (Fars Province) is a favorable area for giardiasis with an anthroponotic infection route.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله محمد ریانی | mohammad rayani
department of microbiology and parasitology, school of health, bushehr university of medical sciences, bushehr, iran and department of medical microbiology and parasitology, faculty of medicine and health sciences, universiti putra malaysia, malaysia.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بوشهر (Bushehr university of medical sciences)

ngah zasmy unyah | ngah zasmy unyah
department of medical microbiology and parasitology, faculty of medicine and health sciences, universiti putra malaysia, malaysia.


غلامرضا حاتم | gholamreza hatam
basic sciences in infectious diseases research center, medical school, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/431
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات