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Iranian Journal of Parasitology، جلد ۹، شماره ۱، صفحات ۹۹-۱۰۶
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عنوان انگلیسی Molecular Detection and Identification of Theileria Species by PCR-RFLP Method in Sheep from Ahvaz, Southern Iran.
چکیده انگلیسی مقاله Background: The present study was carried out to investigate the accurate status of ovine Theileria infection in sheep from Ahvaz and surrounding region, a tropical area southwest Iran. Methods: A PCR-RFLP method based on 18S ribosomal RNA gene was designed which could detect and differentiate Theileria and Babesia spp. and also differentiate main Theileria species in sheep at the same time. 119 sheep blood samples were col-lected from Ahvaz and surroundings. Results: Microscopic examination of blood smears revealed 69.7% (83/119) infec-tion with Theileria spp. Of the total samples subjected to PCR, 89% (106/119) were found to be positive, all of which were identified as Theileria by RFLP analysis using enzyme Hind II. In enzymatic digestion of PCR products by Vsp I, 91.5% (97/106) of Theileria positive samples were identified as T. ovis while mixed Theileria infections were found in 9 samples. The samples with mixed infections were analyzed with an additional nested PCR-RFLP method, by HpaII enzyme digestion. 3 samples with T. lestoquardi infection, 1 sample with T. ovis and T. annulata , 1 sample with T. lestoquardi and T. annulata , and 4 samples with T. ovis , T. lestoquardi and T. annulata mixed infections were detected. Conclusion: Ovine theileriosis caused by T. ovis is highly prevalent in southwest Iran while T. lestoquardi and T. annulata infection can be detected in a lesser propor-tion of sheep in this region. The new PCR-RFLP method that was designed in this study, can serve as a beneficial diagnostic tool, especially in T. ovis prevalent re-gions.
کلیدواژه‌های انگلیسی مقاله 18S rRNA gene,Iran,PCR-RFLP,Sheep,Theileria spp.Babesia
نویسندگان مقاله سیده میثاق جلالی | seyedeh missagh jalali
department of clinical pathology, faculty of veterinary medicine, university of tehran, tehran, iran and department of clinical sciences, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

زهره خاکی | zohreh khaki
department of clinical pathology, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

بهرام کاظمی | bahram kazemi
cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

صادق رهبری | sadegh rahbari
parasitology department, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

پرویز شایان | parviz shayan
parasitology department, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

مژگان بنده پور | mojgan bandehpour
cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

سیده پرستو یاسینی | seyedeh parastoo yasini
department of clinical pathology, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/428
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