این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۸، شماره ۲، صفحات ۱۹۷-۲۰۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Identification and Characterization of the Antigens Expressed On the Surface of Human Erythrocytes Infected With Plasmodium falciparum.
چکیده انگلیسی مقاله Background: Molecules expressed on the surface of infected erythrocytes (IE) with Plasmodium falci-parum play important roles in malaria pathogenesis and immune evasion. Some of these molecules are specific adhesive ligands mediating adhesion of IE to the vascular endothelium. In the current study, the antigens exposed on the surface of IE with different isolates and various binding subpopulations of P. falciparum were studied. Methods: A pooled hyper immune serum (HIS) from Malawian adults and eluted antibodies from the surface of the homologous and heterologous parasites were used. The parasite surface molecules were analyzed by Immuno-Gold-Silver enhancement (IGSE) and Western blotting. Mini-column cytoadherence method was used to select various parasite-binding subpopulations. Results: Surface antigens of all the isolates were recognized by HIS and high recognition of antigens was observed in all isolates with homologous eluted antibodies. Western blot analysis showed that the eluted antibodies reacted with a small subset of antigens compared with HIS. Three bands, Pf EMP-1, were detected in the Triton X- insoluble fraction of the ICAM-1 binding subpopulation. Another interesting band was ~ 52-55 kDa in various isolates of P. falciparum . This molecule as de-fined by its low molecular weight, Triton X-100 solubility, surface location and sensitivity to 1 mg/ml trypsin. Conclusion: The IE's surface antigens differed in parental population compared with the selected subpopulations. These molecules could induce isolate-specific immunity. Antibodies purified from the surface of IE can be used as specific reagents to investigate parasite-derived proteins expressed on the surface of IE.
کلیدواژه‌های انگلیسی مقاله Cytoadherence,PfEMP1,Plasmodium falciparum,Surface antigens
نویسندگان مقاله n کلانتری | n kalantari
cellular and molecular biology research center, babol university of medical sciences; laboratory sciences group, faculty of paramedical, babol university of medical sciences, babol, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)

s غفاری | s ghaffari
parasitology and mycology group, faculty of medicine, babol university of medical sciences, babol, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/505
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات