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Iranian Journal of Parasitology، جلد ۸، شماره ۲، صفحات ۲۸۹-۲۹۵
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عنوان انگلیسی Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan.
چکیده انگلیسی مقاله Background: The present study was designed to detect the presence of tick-borne parasites ( Theileria and Babesia spp . ) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan. Methods: Reverse line blot (RLB) assay was applied for the parasitic detection by the amplification of hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene . A membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified PCR products. Results: Parasites were detected in 16% of the ruminant blood samples under study. Two Theileria species, T. lestoquardi and T. ovis , were identified in samples. 25, of the total 32, infected animals were from Khyber Pukhtoon Khwa. Conclusion : Sheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats ( P > 0.001). Risk factor analysis revealed that male ( P = 0.03), ani-mals infested by ticks ( P = 0.03) and herd composed of sheep only ( P = 0.001) were more infected by blood parasites.
کلیدواژه‌های انگلیسی مقاله 18S rRNA gene,Goat,RLB,Sheep,Theileria,Babesia
نویسندگان مقاله f اقبال | f iqbal
zoology division, institute of pure and applied biology, bahauddin zakariya university multan 60800, pakistan.


rm ختاک | rm khattak
department of zoology, kohat university of science and technology, kohat, pakistan


s ozubek | s ozubek
department of parasitology, faculty of veterinary medicine, university of firat, 23119 elazig, turkey


mnk ختاک | mnk khattak
department of zoology, university of hazara, mansehra, pakistan


a رسول | a rasul
changchun institute of applied chemistry, chinese academy of sciences, changchun 130021, china


m aktas | m aktas
department of parasitology, faculty of veterinary medicine, university of firat, 23119 elazig, turkey
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/493
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