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Iranian Journal of Parasitology، جلد ۸، شماره ۱، صفحات ۶۵-۷۲
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عنوان انگلیسی Potential Use of Biomphalaria alexandrina Snail Antigens for Serodiagnosis of Schistosomiasis Mansoni by Immunoblot Analysis.
چکیده انگلیسی مقاله Background: The aim of this study was to evaluate the possible use of Biomphalaria alexandrina snail antigens in diagnosis of schistosomiasis mansoni using enzyme linked immunolectrotransfere blot (EITB). Methods: S. mansoni adult worm crude antigens (AWA), feet and visceral humps of B. alexandrina and Bulinus truncatus were used. Hyperimmune mice sera (HIS) versus each antigen were prepared for di-agnosis of S. mansoni using western blot (WB). Results: Snail foot antigens were more specific in antibodies detection than visceral hump antigens. Three of five polypeptides of B. alexandrina foot antigen identified by S. mansoni HIS showed specific positive reactivity. These polypeptides were at MW of 31/32 and 43 kDa. While, only one of the six polypeptides of B. alexandrina hepatopancrease antigen identified by S. mansoni HIS, at a MW of 43 kDa was specific. Similarly, 2 polypeptides at MW of 44 and 55 kDa were specific in detection of anti- S. haematobium antibodies. However, the antigenically active polypeptide of B. truncatus hepatopan-crease antigen had no specific reactivity towards anti -S. haematobium antibodies. Conclusion: B. alexandrina foot antigens were the most specific of the tested snail antigens in diagno-sis of schistosomiasis mansoni .
کلیدواژه‌های انگلیسی مقاله EITB,Schistosomiasis mansoni,Snail antigens
نویسندگان مقاله مها ma basyoni | maha ma basyoni
dept. of parasitology, faculty of medicine, cairo university, cairo, egypt.


azza عبد el وهاب | azza abd el wahab
dept. of parasitology, faculty of veterinary medicine, cairo university, cairo, egypt
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/524
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