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Iranian Journal of Parasitology، جلد ۸، شماره ۱، صفحات ۱۰۷-۱۱۳
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عنوان انگلیسی Differential Detection of Echinococcus Spp. Copro-DNA by Nested-PCR in Domestic and Wild Definitive Hosts in Moghan Plain, Iran.
چکیده انگلیسی مقاله Background: Despite Echinococcus granulosus , there are merely two old reports of E. multilocularis in-fection among Iranian canids of Moghan Plain, the only area known endemic for the species. We detected specific DNA markers in fecal samples by PCR (Copro-PCR) for differential diagnosis of Echinococcus species in living canids. Methods: Totally 144 fecal samples from domestic dogs, red foxes and a golden jackal were exam-ined for genus-specific Echinococcus coproantigens using ELISA. Forty two positive or ambiguous samples were further examined for Echinococcus species-specific DNA markers by two different set of nested-PCR. Results: Twenty five out of 144 (17.4%) animals were contaminated with E. granulosus including 14 (23.7%) domestic dogs, 10 (11.9%) red foxes and one (100%) golden jackal. But none of them har-boured E. multilocularis species-specific Copro-DNA. The overall prevalence of E. granulosus and E. multilocularis infections in canids of the area was estimated to be 17.4% and 0.0%, respectively. There was a significant relation between the results of Copro-PCR and CA-ELISA. Conclusion: The lack of E. multilocularis infection, compared to previous reports may be due to the differences in used diagnostic methods and/or recently limited territories of wild canids and altered their food resources in this particular area.
کلیدواژه‌های انگلیسی مقاله Canids,Copro-DNA,Echinococcus,Iran
نویسندگان مقاله i موبدی | i mobedi
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m زارع بیدکی | m zare bidaki
immunology of infectious diseases research center iidrc , rafsanjan university of medical sciences, rafsanjan, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی رفسنجان (Rafsanjan university of medical sciences)

mr سیاوشی | mr siavashi
department of medical parasitology, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

sr نداف | sr naddaf
department of medical parasitology, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

eb کیا | eb kia
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمودی | m mahmoudi
dept. of epidemiology and biostatistics, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/518
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