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Iranian Journal of Parasitology، جلد ۸، شماره ۱، صفحات ۱۶۷-۱۷۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Activity of Superoxide Dismutase (SOD) Enzyme in the Excretory-Secretory Products of Fasciola hepatica and F. gigantica Parasites.
چکیده انگلیسی مقاله Background: The aim of this study was to compare superoxide dismutase (SOD) activity in Fasciola hepatica and Fasciola gigantica parasites. Materials: F. gigantica and F. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °C. Excretory-Secretory (ES) products were collected, centrifuged and stored at - 20 ◦ C. E-S protein concentration was measured by Bradford method and SOD activity was detected using RANSOD kit (Randox Lab. Crumlin, UK). Statistical t -test was conducted for analysis of re-sults. Results: Protein concentration for F. hepatica and F. gigantica were obtained 7.293 ug/ml and 19.65 ug/ml respectively and SOD activity as 0.721 U/ml and 1.189 U/ml , in that order. ES protein con-centration of two species was significantly different ( P < 0.05), however the difference of SOD activi-ty of two species was not significant. Conclusion: Two species of Fasciola have comparable SOD biochemical defense enzyme and can help us explain the parasite survival in host tissue.
کلیدواژه‌های انگلیسی مقاله Excretory-Secretory,Fasciola,Helminth,Superoxide Dismutase
نویسندگان مقاله a فرحناک | a farahnak
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

a گلستانی | a golestani
department of biochemistry, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

mr اشراقیان | mr eshraghian
department of epidemiology and biostatistics, school of public health, tehran university of medical sciences, tehran,.iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/511
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