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Iranian Journal of Parasitology، جلد ۷، شماره ۴، صفحات ۲۲-۲۶
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عنوان انگلیسی Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran
چکیده انگلیسی مقاله B ackground: Acanthamoeba spp . are free-living amoebae commonly found in the environmental sources such as water, soil, and air. This ubiquitous amoeba is the causative agent of amoebic kerati- tis (AK). The objective of the present study was to investigate the presence of Acanthamoeba spp. in water and soil sources in Ahvaz City, Khuzestan Province, southern Iran. M ethods: In general, 110 samples of water and soil were taken from different localities of Ahvaz including agricultural canals, rivers, and swimming pools. Filtration and cultivation were carried out on non-nutrient agar medium (NNA). Axenic cultivation was performed for all of positive isolates. PCR analysis was conducted on positive samples. Sequencing was done for 15 PCR products. Geno - types were identified by Blast search and homology analysis. R esult: Acanthamoeba spp. was found in 43 (71.6%) of samples of water and 13 (26%) soil samples. Genotyping of 15 samples proved that Acanthamoeba belonged to T4 (86.6%), T2 (6.6%), and T5 (6.6%) genotypes. C onclusion: TYI-S-33 medium could be better than PYG medium for Acanthamoeba axenic culture.
کلیدواژه‌های انگلیسی مقاله Acanthamoeba spp.,Water,Soil,TYI-S-33,Iran
نویسندگان مقاله m رهدار | m rahdar
cellular and molecular research center, tropical and infectious diseases research center amp;amp; department of parasitology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

m نیتی | m niyyati
department of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran,iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهدی صالحی | m salehi
cellular and molecular research center, tropical and infectious diseases research center amp;amp; department of parasitology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

m فقهی | m feghhi
department of ophthalmology, imam khomeini hospital, ahvaz jundishapur university of medical sciences, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

m مکوندی | m makvandi
department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

m پورمهدی | m pourmehdi
department of epidemiology and statistical, school of veterinary, shahid chamran university, ahvaz, iran.

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

s فرنیا | s farnia
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran,iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/280
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